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pJT175_GalL_A3A(Y130F)Δ186-BE3

Plasmid

#145124

Purpose

Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(Y130F)Δ186-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

A3A(Y130F; 1-186AA); spCas9(D10A)

Promoter

GalL

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-dnTCF4

Plasmid

#114277

Purpose

TET-inducible expression of a dominant negative form of the TCF4 transcription factor

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

TCF4 (TCF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

deletion of AA 1 to 31

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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CROPseq-MPSV-eBFP2

Plasmid

#220472

Purpose

Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

Depositor

Christian Krebs

Article

Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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RLuc_MET_95_FLuc

Plasmid

#226463

Purpose

MET predicted IRES between Rluc and Fluc

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-Exon1-MET

Plasmid

#216535

Purpose

MET CDS with 5'UTR

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET with 5'UTR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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RLuc_MET_95_FLuc_Pless

Plasmid

#226464

Purpose

Promoterless MET predicted IRES between Rluc and Fluc

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

Predicted MET IRES

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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RLuc_IRES_FLuc_Pless

Plasmid

#226462

Purpose

Promoterless bicistronic luciferase

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

RLuc IRES FLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

No promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-uORF1,2-MET

Plasmid

#214106

Purpose

MET 5'UTR upstream of mGFP (uORF1 and uORF2 mutated)

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR uORF1 and 2 mutated

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-Exon2-MET

Plasmid

#216536

Purpose

MET CDS without 5'UTR

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET without 5'UTR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF1

Plasmid

#216537

Purpose

gRNA and template for MET 5'UTR uORF1 mutation

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF1 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF2

Plasmid

#216538

Purpose

gRNA and template for MET 5'UTR uORF2 mutation

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF2 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_5'UTR_MET_GFP

Plasmid

#214103

Purpose

MET 5'UTR upstream of mGFP

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-mCherry-CAAX

Plasmid

#218189

Purpose

To over express mCherry-CAAX under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

mCherry-CAAX

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSUPER TBK1

Plasmid

#26210

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER TBK1 (TBK1 Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pSF11-wmCardinal

Plasmid

#197251

Purpose

Expresses the protein of wmCardinal in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-uORF2-MET

Plasmid

#214105

Purpose

MET 5'UTR upstream of mGFP (uORF2 mutated)

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR uORF2 mutated

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.23[METUTRwt/luc2/minP]

Plasmid

#226458

Purpose

wt MET 5'UTR upstream FLuc

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.23[uORF1/luc2/minP]

Plasmid

#226459

Purpose

MET 5'UTR (uORF1 mutated) upstream FLuc

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR uORF1 mutated

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.23[uORF2/luc2/minP]

Plasmid

#226460

Purpose

MET 5'UTR (uORF2 mutated) upstream FLuc

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR uORF2 mutated

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-uORF1-MET

Plasmid

#214104

Purpose

MET 5'UTR upstream of mGFP (uORF1 mutated)

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

uORF1 mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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