Addgene

We narrowed to 664 results for: germes

Showing: 121 - 140 of 664 results

Results per page:

Sort results by:

pLKO.1-Teton-puro-shPP1C #2

Plasmid

#198763

Purpose

conditional knockdown of PP1C

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shPP1C #2 (PPP1CC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-Teton-puro-shPP1C #1

Plasmid

#198762

Purpose

conditional knockdown of PP1C

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shPP1C #1 (PPP1CC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-Yap1-E1-#2

Plasmid

#171519

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-BE3-NLS

Plasmid

#119030

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-Teton-puro-shOCT4

Plasmid

#198761

Purpose

conditional knockdown of OCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shOCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-FLAG-IRES-Blasticidin-hOCT4 WT

Plasmid

#198764

Purpose

Mammalian expression of hOCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 WT (POU5F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

Plasmid

#198765

Purpose

expression of hOCT4 mutants

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 236D (POU5F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S236D

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnos-Fok1:dCas9-nos

Plasmid

#62210

Purpose

Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Insert

Fok1-dCas9

Use

Tags

Expression

Insect

Mutation

Promoter

nanos

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
delta-BRIP1-pcDNA3.1/MycHis

Plasmid

#19042

Depositor

Ronny Drapkin

Article

De Nicolo et al Clin Cancer Res. 2008 Jul 15. 14(14):467

Insert

delta BRIP1 (BRIP1, BRCA1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

This is a novel BRIP1 germline mutation that cons…

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pRVV210

Plasmid

#87628

Purpose

Drives Bxb1 expression in the D. melanogaster germ line.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVV655

Plasmid

#119019

Purpose

Drives TP901-1 recombinase expression in the D. melanogaster germ line

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 recombinase

Use

Tags

SV40 NLS

Expression

Insect

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0285

Plasmid

#162290

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0284

Plasmid

#162289

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
NmCherry-SpVasa

Plasmid

#66786

Purpose

expression of mCherry in Sea Urchin germ cells

Depositor

Amro Hamdoun

Article

Campanale et al Development. 2012 Feb;139(4):783-92. doi

Insert

Vasa

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pEPQDKN0332

Plasmid

#162314

Purpose

Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

SP6prom-EO1-sfGFP-HiBiT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CB0026

Plasmid

#162285

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CB0246

Plasmid

#162286

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0283

Plasmid

#162288

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0282

Plasmid

#162287

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaster1

Plasmid

#58525

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 )
myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
Nanog (NANOG Human)
IRES

Use

Tags

Expression

Mutation

Promoter

CAG and EF1a

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge