We narrowed to 596 results for: pFAST

1. pfast bac Rbx-HA

   Plasmid

   #51893

   Purpose

   Backbone plasmid is pfastBacHTa from invitrogen

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   Rbx1 (RBX1 Human)
   

   Tags

   HA

   Promoter

   polyhedrin promoter

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pFastBac His HA GNU WT

   Plasmid

   #112994

   Purpose

   Baculovirus expression of His/HA-tagged Drosophila gnu

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

   Insert

   gnu (gnu Fly)
   

   Tags

   His/HA

   Expression

   Insect

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac Mel18 flag

   Plasmid

   #1969

   Depositor

   Robert Kingston

   Article

   Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

   Insert

   Mel18 (Pcgf2 Mouse)
   

   Tags

   flag

   Expression

   Insect

   Available Since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pFastBac-LacI-LAP

   Plasmid

   #114034

   Purpose

   Production of baculovirus with LacI-LAP

   Depositor

   Geert Kops

   Article

   Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

   Insert

   LacI-LAP
   

   Expression

   Insect

   Mutation

   none

   Promoter

   CMV

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-hDcr-K70A.

   Plasmid

   #89145

   Purpose

   Expression of helicase mutant of human Dicer in Sf9 cells

   Depositor

   Jennifer Doudna

   Article

   MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

   Insert

   hDicer (DICER1 Human)
   

   Tags

   His6-tev

   Expression

   Insect

   Mutation

   K70A

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac SOX R139A

   Plasmid

   #192058

   Purpose

   Expression vector for SOX (ORF37) R139A

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (ORF37)
   

   Expression

   Insect

   Mutation

   R139A

   Promoter

   Polyhedrin

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac SOX Q129H

   Plasmid

   #192057

   Purpose

   Expression vector for SOX (ORF37) Q129H

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (ORF37) Q129H
   

   Expression

   Insect

   Mutation

   Q129H

   Promoter

   Polyhedrin

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1-NEDD4L_ΔC2 (157)

   Plasmid

   #187720

   Purpose

   Express GST-tagged NEDD4L ΔC2 (157) in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 157-975 (NEDD4L Human)
   

   Tags

   GST-HRV 3C site

   Expression

   Insect

   Mutation

   deletion of residues 1-156

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

   Plasmid

   #187723

   Purpose

   Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 1-418+582-975 (NEDD4L Human)
   

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   deletion of residues 419-581

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBacHTA-NEDD4L_W1-L-W2-HECT

    Plasmid

    #187724

    Purpose

    Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 153-418+582-975 (NEDD4L Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    deletion of residues 1-152 and 419-581

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBacI-KDAC6 H216A

    Plasmid

    #224351

    Purpose

    Expresses human KDAC6 (HDAC6) H216A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

    Insert

    KDAC6 (HDAC6 Human)
    

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 216 mutated to alanine

    Promoter

    Polyhedrin

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pFastBacI-KDAC6 H611A

    Plasmid

    #224352

    Purpose

    Expresses human KDAC6 (HDAC6) H611A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

    Insert

    KDAC6 (HDAC6 Human)
    

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 611 mutated to alanine

    Promoter

    Polyhedrin

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pFastBac-GST-TBK1

    Plasmid

    #221331

    Purpose

    TBK1 protein overexpression in Sf9 insect cells

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    TBK1 (Tbk1 Mouse)
    

    Tags

    6xHis-TEV-2xGST-TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac1-C17orf53-Strep

    Plasmid

    #211446

    Purpose

    generation of Bacmid for insect cell expression of C17orf53 C-terminally fused to Strep-Tag

    Depositor

    Helmut Pospiech

    Insert

    C17orf53 (HROB Human)
    

    Tags

    Strep-Tag

    Expression

    Insect

    Available Since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac1-C17orf53-FLAG

    Plasmid

    #211447

    Purpose

    generation of Bacmid for insect cell expression of C17orf53 C-terminally fused to FLAG-Tag

    Depositor

    Helmut Pospiech

    Insert

    C17orf53 (HROB Human)
    

    Tags

    FLAG-Tag

    Expression

    Insect

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac1-bullfrog saxiphilin

    Plasmid

    #140595

    Purpose

    protein expression

    Depositor

    Dan Minor

    Article

    Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

    Insert

    saxiphilin bullfrog
    

    Tags

    GFP-His10

    Expression

    Insect

    Mutation

    Deletion of M20 - please see depositor comment

    Promoter

    polyhedrin promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac FAK W266A

    Plasmid

    #186144

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    DNA mutation T796G;G797C Amino acid mutation W266A

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG660 lyn11-pFAST

    Plasmid

    #172863

    Purpose

    expresses lyn11-pFAST in mammalian cells (inner membrane labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    Lyn11-pFAST
    

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1_GI.1_A37C-A44C

    Plasmid

    #162582

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Alanine 37 changed to Cysteine and Alanine 44 cha…

    Promoter

    polyhedring

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac1_GI.1_A37I-A44L

    Plasmid

    #162583

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    A37I and A44L

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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