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We narrowed to 651 results for: pFAST

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Showing: 121 - 140 of 651 results

  1. pFastBac HT JS-Munc18b

    Plasmid
    #135554
    Purpose
    Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.
    Depositor
    Jingshi Shen
    Publication
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18b (Stxbp2 Rat)
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available since
    March 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    135554-plasmid-map-sequence-id-272494

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  3. 891 pFastBac GST PTEN

    Plasmid
    #10937
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    GST
    Expression
    Insect
    Available since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  5. pFastBacDual with LFn + FlaAAA

    Plasmid
    #84867
    Purpose
    Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Publication
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    FlaAAA
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    Polyhedrin
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    84867-plasmid-map-sequence-id-162897

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  6. pFastBacHTa_LYN

    Plasmid
    #224244
    Purpose
    Expression of human LYN Kinase in insect cells (SF9 cells)
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Tags
    6xHis-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224244-plasmid-map-sequence-id-458379

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  7. pFAST-His-mEGFP-TEV-Abl-WT

    Plasmid
    #258178
    Purpose
    Used for generation of bacmid DNA and subsequent baculovirus for insect cell expression/purification of Abl-WT.
    Depositor
    Lindsay Case
    Insert
    ABL1 (ABL1 Human)
    Tags
    his(6)mEGFP
    Expression
    Insect
    Promoter
    polh
    Available since
    Aug. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    258178-plasmid-map-sequence-id-515002

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  8. pFAST-His-mEGFP-TEV-Abl-RS

    Plasmid
    #258179
    Purpose
    Used for generation of bacmid DNA and subsequent baculovirus for insect cell expression/purification of Abl-RS.
    Depositor
    Lindsay Case
    Insert
    ABL1 (ABL1 Human)
    Tags
    his(6)mEGFP
    Expression
    Insect
    Mutation
    All 33 arginines in the intrinsically disordered …
    Promoter
    polh
    Available since
    Aug. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    258179-plasmid-map-sequence-id-514996

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  9. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid
    #64064
    Purpose
    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
    Depositor
    Andrew Carter
    Publication
    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi
    Insert
    Cytoplasmic dynein-2 (DYNC2H1 Human)
    Tags
    GFP, His tag, and ZZ tag
    Expression
    Insect
    Mutation
    Deleted amino acids 1-1090, extra C-terminal vali…
    Promoter
    Polyhedrin
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64064-plasmid-map-sequence-id-110915

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  14. pFastBacI/MDR3cysless

    Plasmid
    #74400
    Purpose
    expression of MDR3 in insect cells
    Depositor
    Robert Nakamoto
    Insert
    MDR3 (Abcb1a Mouse)
    Tags
    10His
    Expression
    Insect
    Mutation
    all Cys to Ala
    Promoter
    Polyhedrin
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 121 - 140 of 651 results