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We narrowed to 275 results for: plasmid transfection

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Showing: 121 - 140 of 275 results

  1. pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

    Plasmid
    #154770
    Purpose
    plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, G1 PylT with A41AA and C5…
    Promoter
    EF1
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154770-plasmid-map-sequence-id-309867

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  2. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

    Plasmid
    #154778
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    LaG17-SynNotch
    Tags
    LaG17
    Expression
    Mammalian
    Mutation
    204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…
    Promoter
    EF1
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154778-plasmid-map-sequence-id-310407

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  3. qTAG-N-Solo-mStayGold-CENPA

    Plasmid
    #227283
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227283-plasmid-map-sequence-id-451297

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  4. qTAG-C-mStayGold-EFS-Blast-ARL13B

    Plasmid
    #227278
    Purpose
    Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227278-plasmid-map-sequence-id-451732

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  5. qTAG-C-moxGFP-Puro-LAMP1

    Plasmid
    #207788
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207788-plasmid-map-sequence-id-416383

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  6. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid
    #207792
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207792-plasmid-map-sequence-id-413795

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  7. qTAG-C-Solo-mStayGold-TOMM20

    Plasmid
    #227307
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227307-plasmid-map-sequence-id-451546

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  8. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid
    #227322
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227322-plasmid-map-sequence-id-452101

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  9. qTAG-N-Solo-mStayGold-CETN2

    Plasmid
    #227292
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227292-plasmid-map-sequence-id-452065

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  10. qTAG-N-Solo-mScarlet-LMNB1

    Plasmid
    #207771
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207771-plasmid-map-sequence-id-416246

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  11. qTAG-N-Puro-mStayGold-LMNB1

    Plasmid
    #227329
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227329-plasmid-map-sequence-id-451729

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  12. qTAG-N-Solo-mStayGold-ACTB

    Plasmid
    #227326
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227326-plasmid-map-sequence-id-452114

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  13. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227321-plasmid-map-sequence-id-452094

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  14. qTAG-N-Blast-dTAG-LMNB1

    Plasmid
    #207776
    Purpose
    Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207776-plasmid-map-sequence-id-416195

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  15. qTAG-C-Solo-mStayGold-VIM

    Plasmid
    #227302
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    VIM Homology Arms flanking a mStayGold Tag (VIM Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227302-plasmid-map-sequence-id-452073

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  16. qTAG-C-Solo-mStayGold-CANX

    Plasmid
    #227280
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CANX Homology Arms flanking a mStayGold Tag (CANX Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227280-plasmid-map-sequence-id-452047

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  17. qTAG-C-miRFP670nano3-Blast-H3C2

    Plasmid
    #207784
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207784-plasmid-map-sequence-id-416184

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  18. qTAG-N-Blast-mScarlet-ACTB

    Plasmid
    #207753
    Purpose
    Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207753-plasmid-map-sequence-id-416363

    Enlarge

  19. qTAG-C-Solo-mStayGold-MAPRE1

    Plasmid
    #227324
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAPRE1 Homology Arms flanking a mStayGold Tag (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227324-plasmid-map-sequence-id-452106

    Enlarge

  20. qTAG-N-Solo-mStayGold-CEP135

    Plasmid
    #227287
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP135 Homology Arms flanking a mStayGold Tag (CEP135 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227287-plasmid-map-sequence-id-452057

    Enlarge

Showing: 121 - 140 of 275 results