We narrowed to 134 results for: spycatcher
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Plasmid#186908PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)2 linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)2-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch
Plasmid#186906PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-1xL
Plasmid#186907PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-TMD
Plasmid#195347PurposeFluorescent labeling for cell membranesDepositorInsertHaloTag
ExpressionMammalianPromoterCMVAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-H-Lock
Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
TagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialPromoterT7Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(M115G)-Talin rod-mCherry
Plasmid#210026PurposeExpression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry
ExpressionMammalianMutationSpyTag003 M115G mutation to modify SpyCatcher003 …PromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(M115A)-Talin rod-mCherry
Plasmid#210025PurposeExpression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry
ExpressionMammalianMutationSpyTag003 M115A mutation to modify SpyCatcher003 …Available SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(V114T, V116T)-Talin rod-mCherry
Plasmid#210027PurposeExpression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
ExpressionMammalianMutationSpyTag003 V114T and V116T mutations to modify Spy…PromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only