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MARK3 gRNA (BRDN0001147831)

Plasmid

#77718

Purpose

3rd generation lentiviral gRNA plasmid targeting human MARK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MARK3 (MARK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgMARK3

Plasmid

#138706

Purpose

Expresses a human MARK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK3 human (MARK3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-Caveolin1-CMV-GFP

Plasmid

#83272

Purpose

Adenoviral expression of Caveolin1 with co-expression of GFP

Depositor

Andrew Brooks

Article

Fernandez-Rojo et al Cell Rep. 2013 Jul 25;4(2):238-47. doi:

Inserts

CAV1 (CAV1 Human)
eGFP

Use

Adenoviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-MARK3

Plasmid

#23716

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MARK3 (MARK3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLT3REVIR_MARK3 2573

Plasmid

#107233

Purpose

shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLT3REVIR_MARK3 1122

Plasmid

#107232

Purpose

shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12039 - pAAV-3xSP10ins-AiE0354h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2039)

Plasmid

#208401

Purpose

AiE0354h is an enhancer sequence designed to drive AAV-mediated transgene expression in Vip+ cortical interneurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Lee et al Science. 2023 Oct 13;382(6667):eadf6484.

Insert

SYFP2

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

eHGT_354h (enhancer)

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12040 - pAAV-3xSP10ins-AiE0354m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2040)

Plasmid

#223971

Purpose

AiE0354m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14908 - pAAV-AiE0354h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4908)

Plasmid

#224052

Purpose

AiE0354h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

mScarlet

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15262 - pAAV-AiE0219h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5262)

Plasmid

#224062

Purpose

AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

mScarlet

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15295 - pAAV-AiE0219h_3xC3-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5295)

Plasmid

#224055

Purpose

AiE0219h_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12565 - pAAV-3xSP10ins-AiE0354m_3xC2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2565)

Plasmid

#223994

Purpose

AiE0354m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12293 - pAAV-3xSP10ins-AiE0219h_3xC2-minRho-SYFP2-8X2C-WPRE3-BGHpA (Alias: CN2293)

Plasmid

#223980

Purpose

AiE0219h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15265 - pAAV-AiE0219h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5265)

Plasmid

#224054

Purpose

AiE0219h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12850 - pAAV-AiE0659m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2850)

Plasmid

#224010

Purpose

AiE0659m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge