Addgene

We narrowed to 14,720 results for: nans

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pSA101

Plasmid

#59787

Purpose

drives ZIF-1 protein in C. elegans neurons

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

rab-3 promoter
zif-1 coding (zif-1 Nematode)
unc-54 3' UTR

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GeNL

Plasmid

#101002

Purpose

Construct for fusion expression of GeNL reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

Expression

Mutation

Promoter

Ribi

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pFtnA-Ypet

Plasmid

#98280

Purpose

mammalian expression of FtnA-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSNAP-FtnA

Plasmid

#98282

Purpose

mammalian expression of SNAP-FtnA

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Use

Tags

SNAPf-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCuGFP-SpAtg17(416)

Plasmid

#122080

Purpose

Expressing S. pombe GFP-Atg17

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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POC1468

Plasmid

#213756

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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POC1467

Plasmid

#213755

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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B2401_miRFP-FKBP5x

Plasmid

#215118

Purpose

Expresses miRFP-FKBP-FKBP-FKBP-FKBP-FKBP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

miRFP-FKBP-FKBP-FKBP-FKBP-FKBP

Use

Tags

miRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEPCTαKN0013

Plasmid

#187564

Purpose

Transcriptional unit for copper-inducible expression of nanoluc luciferase driven by a minimal synthetic promoter with binding sites for CUP2

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Luciferase

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEPCTΩSP0017

Plasmid

#187565

Purpose

Module for constitutive expression of firefly luciferase and nanoluc luciferase driven by 35s promoters

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Firefly Luciferase

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCuHA-SpAtg17(416)

Plasmid

#122079

Purpose

Expressing S. pombe HA-Atg17

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-SpAtg17(416)

Plasmid

#122078

Purpose

Expressing S. pombe Atg17

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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Actin-OptoNB

Plasmid

#159595

Purpose

Opto-Nanobody

Depositor

Jared Toettcher, Jose Avalos

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

ActinNB with AsLOV2 at loop 5 with 62-666

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-ARS-stacked-NeoR-mNeonGreen

Plasmid

#101249

Purpose

Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

Expression

Mutation

Promoter

Ribi

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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APEX2-csChBP

Plasmid

#108876

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csChBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMSP1E2D1

Plasmid

#161606

Purpose

Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Insert

MSP1E2D1

Use

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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NbV5-LgBit

Plasmid

#201475

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-LgBit

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEff

Plasmid

#97423

Purpose

Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

Depositor

Nikolai Ravin

Article

Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pTXB1-nbOcIgG-Tn5

Plasmid

#184285

Purpose

Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

New York Genome Center, Ivan Raimondi

Article

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbOcIgG-Tn5

Use

Tags

Expression

Mutation

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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