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SpyCatcher-DIIG

Plasmid

#201196

Purpose

Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-DIIG

Tags

HisTag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-SWG

Plasmid

#201194

Purpose

Expresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-SWG

Tags

HisTag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-PEED

Plasmid

#201195

Purpose

Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-PEED

Tags

HisTag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-43

Plasmid

#172759

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-43

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-35

Plasmid

#172755

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-35

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-19

Plasmid

#172748

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-19

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-3

Plasmid

#172740

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-12

Plasmid

#172744

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-12

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-14

Plasmid

#172745

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-14

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

pCMV and T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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SaLgCG

Plasmid

#164563

Purpose

GFP

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP29[dCas9]-1A(lacZ)

Plasmid

#126979

Purpose

Level 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

Cas9 catalytically dead

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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TIAM2_PDZ_1

Plasmid

#103952

Purpose

Protein expression and purification of TIAM2 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TIAM2 (TIAM2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB(SnaBI)

Plasmid

#69558

Purpose

In vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

The two last codons (TATTAA; encoding the last ty…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBB(Y305P)

Plasmid

#69557

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

Codon TAT encoding tyrosine 305 was changed to CC…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pED55 pRS306 pRPS2-SteND-NLS-mCherry

Plasmid

#68277

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7ND NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

Plasmid

#68275

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS(4A) mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET14b-PITPalpha N90F

Plasmid

#58257

Purpose

bacterial expression of human PITP alpha N90F

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Tags

HIS

Expression

Bacterial

Mutation

N90F (abolishes PI binding)

Promoter

T7

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pET14b-PITPalpha K61A

Plasmid

#58258

Purpose

bacterial expression of human PITP alpha K61A

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I L30&37P

Plasmid

#22166

Depositor

Ananda Roy

Article

Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Mutation

Changed Leucine 30 to Proline and changed leucine…

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only