We narrowed to 10,938 results for: AGA

1. BC-A1-008

   Plasmid

   #78695

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
   

   Use

   Synthetic Biology

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. BC-A1-007

   Plasmid

   #78694

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. shCDK4(1)

   Plasmid

   #73550

   Purpose

   shRNA(1) against CDK4

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pUAST attB BmOr2 (Y464A)

   Plasmid

   #73927

   Purpose

   UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to Alanine

   Depositor

   Leslie Vosshall

   Article

   Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

   Insert

   BmOr2 (Y464A)
   

   Expression

   Insect

   Mutation

   changed 464 Tyrosine to Alanine

   Promoter

   hsp70

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO1-puro-U6-sgRNA-TS4

   Plasmid

   #69236

   Purpose

   U6 driven SpCas9 sgRNA expression for TS4 site

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS4 sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHL671

   Plasmid

   #62825

   Purpose

   Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Insert

   gfp
   

   Use

   Synthetic Biology

   Tags

   glgC leader

   Expression

   Bacterial

   Promoter

   pCon

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pHL1017

   Plasmid

   #62828

   Purpose

   Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Insert

   gfp
   

   Use

   Synthetic Biology

   Tags

   m8::glgC leader

   Expression

   Bacterial

   Promoter

   pConNoHind

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET11+PCNA3-CYP119

   Plasmid

   #66136

   Purpose

   Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

   Insert

   CYP119 (cyp119 )
   

   Tags

   Sulfolobus solfataricus PCNA3

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET11+PCNA3-CYP119(T214V)

   Plasmid

   #66137

   Purpose

   Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

   Insert

   CYP119 (cyp119 )
   

   Tags

   Sulfolobus solfataricus PCNA3

   Expression

   Bacterial

   Mutation

   T214V

   Promoter

   T7 promoter

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pASKt15C+SrtA5woH

    Plasmid

    #65019

    Purpose

    Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTH1-SaSrtA-KQ

    Plasmid

    #64982

    Purpose

    Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108Q

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nano-lantern(Ca2+)_63/pRSETB

    Plasmid

    #51980

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-4GS
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pattB-3xFlag-Loqs-PA

    Plasmid

    #41108

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    Loquacious-PA (loqs Fly)
    

    Tags

    3xFlag

    Expression

    Insect

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLenti-CMV-cGAS-HA

    Plasmid

    #130910

    Purpose

    Expresses human cGAS-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJAK184

    Plasmid

    #167281

    Purpose

    MazF-based mutagenesis vector for C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Promoter

    Pxyl

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJAK080

    Plasmid

    #167279

    Purpose

    CodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge