Addgene

We narrowed to 21,471 results for: ESC;

Showing: 1381 - 1400 of 21471 results

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LD124

Plasmid

#69174

Purpose

Integration of GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg24-EGFP

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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LD120

Plasmid

#69171

Purpose

Integration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg20-EGFP

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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Phamret-Beta-Catenin-20

Plasmid

#57753

Purpose

Localization: Tight Junctions, Excitation: 458 / 475, Emission: 458 / 517

Depositor

Michael Davidson

Insert

Beta-Catenin (Ctnnb1 Mouse)

Use

Tags

Phamret

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Use

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN

Plasmid

#28298

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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FUGW-PercevalHR

Plasmid

#49083

Purpose

Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMBI

Plasmid

#17816

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
GW1-pHRed

Plasmid

#31473

Purpose

genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA3mt

Plasmid

#140464

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA3mt

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA4mt

Plasmid

#140463

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA4mt

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-deadMalibu-mScarlet

Plasmid

#229555

Purpose

Fluorescent reporter for malonyl-CoA that does not bind malonyl-CoA (control)

Depositor

Danielle Schmitt

Article

Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

Insert

Malibu-mScarlet

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxOFP

Plasmid

#229950

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Article

Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

Insert

luxOFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxNeon

Plasmid

#229947

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

Insert

luxNeon

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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ilux pGEX(-)

Plasmid

#107879

Purpose

Bacterial expression of ilux for bioluminescence imaging

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

Insert

ilux

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTW357b

Plasmid

#227930

Purpose

Low-stringency accessory plasmid for selection

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Use

Tags

Expression

Bacterial

Mutation

R632S

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-mScarlet-WPRE

Plasmid

#99280

Purpose

Can be used to generate AAV virus that will express mScarlet in the presence of Cre

Depositor

Rylan Larsen

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pOPH6

Plasmid

#40315

Depositor

Shaun Lott

Article

Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

Insert

Peptide:N-glycosidase F

Use

Tags

6xHis and OmpA3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pTW357d5

Plasmid

#227931

Purpose

High-stringency accessory plasmid for selection

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Use

Tags

Expression

Bacterial

Mutation

R632S, Q649S

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-GFP

Plasmid

#58867

Purpose

AAV expression of GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Syn

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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