We narrowed to 3,284 results for: cgas

1. pAG306-pTet07-synYFP[TTG/AGA]-nLuc

   Plasmid

   #199756

   Purpose

   Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

   Depositor

   Brian Zid

   Article

   Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

   Insert

   synYFP[TTG/AGA]-nLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-PKCG

   Plasmid

   #16180

   Depositor

   Huda Zoghbi

   Article

   Lim et al Cell. 2006 May 19. 125(4):801-14.

   Insert

   PRKC gamma (PRKCG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pM115: CasRx control presgRNA

   Plasmid

   #166867

   Purpose

   U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   non-targeting presgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-mEGFP-PKCg-C1

   Plasmid

   #112266

   Purpose

   ITRACKgamma Donor Construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   protein kinase c gamma (Prkcg Mouse)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV/3' Box_(GLuc)_TOP2

   Plasmid

   #68435

   Purpose

   Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP2 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6_(Gluc)_INT(GenPurpClon)

   Plasmid

   #68433

   Purpose

   General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct
   

   Use

   CRISPR; Cloning vector for generating int-like co…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6_(GLuc)_INT[Spinach2]

   Plasmid

   #68430

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Spinach2" aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL-mU6-GFPGO-IRES-mScarletI

   Plasmid

   #136895

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO-IRES-mScarletI
   

   Use

   Lentiviral

   Tags

   3xNLS on mScarlet

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-hU6-mU6-Cre2GO

   Plasmid

   #136907

   Purpose

   Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   BlasCyGO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid

    #70656

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pABM-D4-miR30-L1221

    Plasmid

    #132933

    Purpose

    Lentivector for luciferase (control) knockdown and blasticidin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    luciferase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6_(GLuc)_TOP1

    Plasmid

    #68423

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOC5 Tubb3-GFP KI

    Plasmid

    #183440

    Purpose

    FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCol-TGM-luc.1309

    Plasmid

    #32720

    Depositor

    Scott Lowe

    Article

    Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

    Insert

    luciferase
    

    Use

    Mouse Targeting and RNAi

    Tags

    Expression

    Mutation

    Promoter

    TRE and Tre

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pAN-PBAD-sgRNA-scramble

    Plasmid

    #62285

    Purpose

    expression of scramble sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    scramble sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pG3H-U6EC1

    Plasmid

    #134758

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-EC1

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPU-Empty-miR30-L1221

    Plasmid

    #132940

    Purpose

    All-in-one control rescue-control shRNA knockdown vector

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPU-huTRIMmiR30-L1221

    Plasmid

    #132937

    Purpose

    All-in-one huTRIM5 rescue-control shRNA knockdown

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    human TRIM5
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330_ATP1A1_G7

    Plasmid

    #173204

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CROP_C9_Puro_SRC

    Plasmid

    #183322

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets SRC gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    SRC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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