We narrowed to 5,554 results for: donor

1. AAVS1-Puro cTnT CCND2-T2A-ChloC

   Plasmid

   #179841

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR and TALEN; Donor plasmid

   Tags

   chloride-conducting ChR (ChloC)

   Expression

   Mammalian

   Promoter

   Troponin T promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-Puro cTnT CCND2-T2A-iChloC

   Plasmid

   #179842

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR and TALEN; Donor plasmid

   Tags

   improved chloride-conducting ChR (iChloC)

   Expression

   Mammalian

   Promoter

   Troponin T promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Puro cTnT CCND2-T2A-luciferase

   Plasmid

   #179844

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR, Luciferase, and TALEN ; Donor plasmid

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Troponin T promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pS12<ActB.mus_2A-mCh_KI-HMEJ

   Plasmid

   #207407

   Purpose

   Donor template to knock in mCherry at the C-terminus of mouse ActB (β-Actin) using DSB repair by homology-mediated end joining (HMEJ). [Lab plasmid ID: TU148]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   Actb (Actb Mouse)
   

   Use

   CRISPR and Mouse Targeting; Hmej knockin donor

   Tags

   mCherry

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMMDS 2 colors

   Plasmid

   #206265

   Purpose

   Expression of 2 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   GST mTagBFP1 (Synthetic), Ctnnb1 (M. musculus) (Ctnnb1 )
   

   Use

   Cre-donor

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MultiMate-HITI-2c ACTB

   Plasmid

   #206267

   Purpose

   All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
   

   Use

   CRISPR; Recombinant baculovirus production

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMMK ENTR 1 HITI-2c ACTB

   Plasmid

   #206269

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   HITI-2c donor
   

   Use

   CRISPR; Multimate/gateway entr 1

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-puro-U6-NmeCas9-TLR-MCV1-sgRNA

   Plasmid

   #117407

   Purpose

   NmeCas9 sgRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   NmeCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-puro-U6-CjeCas9-TLR-MCV1-sgRNA

   Plasmid

   #117408

   Purpose

   CjeCas9 sgRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   CjeCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-puro-U6-SauCas9-TLR-MCV1-sgRNA

    Plasmid

    #117409

    Purpose

    SauCas9 sgRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SauCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNA

    Plasmid

    #117410

    Purpose

    LbCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    LbCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-

    Plasmid

    #191390

    Purpose

    Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(1-100)Cy-
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GK Rab6A p201

    Plasmid

    #44700

    Depositor

    Geri Kreitzer

    Insert

    Rab6A (RAB6A Human)
    

    Use

    Gateway donor vector

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pFETCh_ZNF3

    Plasmid

    #72362

    Purpose

    Donor vector for 3' FLAG tag of human ZNF3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    ZNF3 Homology Arms (ZNF3 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. GK Rab11A p201

    Plasmid

    #44704

    Depositor

    Geri Kreitzer

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Gateway donor vector

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pPV476

    Plasmid

    #42929

    Depositor

    James Lok

    Insert

    attR2::egfp (870 bp)::taa::Ss-era-1 terminator (598 bp)::attL3
    

    Use

    Multi-site gateway donor plasmid

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rhesus AAVS1-CAG-copGFP

    Plasmid

    #84209

    Purpose

    Rhesus AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP

    Depositor

    Cynthia Dunbar

    Inserts

    Rhesus AAVS1 5’ homology arm
    Rhesus AAVS1 3’ homology arm
    

    Expression

    Mammalian

    Available Since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid

    #191529

    Purpose

    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    H2BK_mNG_hSLBP(18-108)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mNeonGreen (mNG)

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. GK Rab6A’ T27N p201

    Plasmid

    #44702

    Depositor

    Geri Kreitzer

    Insert

    Rab6A' (RAB6A Human)
    

    Use

    Gateway donor vector

    Mutation

    T27N

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFHMSPN-avi-TEV-LIC V

    Plasmid

    #220885

    Purpose

    Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus expresssion

    Tags

    Honeybee melittin signal followed by His, avi and…

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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