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Gfa104-hIRβ

Plasmid

#236227

Purpose

Expressing a truncated, constitutively active human insulin receptor (IRβ) in astrocyte

Depositor

Olivier Thibault

Article

Frazier et al Neurochem Res. 2019 Jan;44(1):269-280. d

Insert

human Insulin Receptor beta subunit (INSR Human)

Use

AAV

Expression

Bacterial

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304_miRFP670nano-P2A-ERM-gCam6f

Plasmid

#214927

Purpose

Express miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)

Depositor

Xinnan Wang

Article

Bharat et al Cell Rep. 2023 Dec 26;42(12):113544. doi

Insert

miRFP670nano-P2A-ERM-gCam6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMVp

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(172-554)

Plasmid

#183664

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 172-554

Promoter

CMV

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(K525A)

Plasmid

#183659

Purpose

N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

K525A

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(K528A)

Plasmid

#183660

Purpose

N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

K528A

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(33-520)

Plasmid

#183662

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 33-520

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(211-554)

Plasmid

#183665

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 211-554

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(C460S)

Plasmid

#183658

Purpose

N-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

C460S

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B Q116P G120

Plasmid

#129291

Purpose

Gateway entry clone encoding human deconjugation-resistant LC3B

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway Cloning

Mutation

Changed Glutamine 116 to Proline. Deleted amino a…

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_1-590 POLI

Plasmid

#131233

Purpose

Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_1-420 POLI

Plasmid

#131234

Purpose

Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-7 aSyn C141

Plasmid

#108866

Purpose

Expresses human WT alpha synuclein with a C-terminal cysteine

Depositor

Gabriele Kaminski Schierle

Article

Stephens et al Commun Biol. 2023 May 15;6(1):526. doi:

Insert

alpha synuclein (SNCA Human)

Expression

Bacterial

Mutation

C141 mutation to allow C-terminal maleimide dye l…

Promoter

T7

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3B Q116P G120

Plasmid

#129289

Purpose

Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Changed Glutamine 116 to Proline. Deleted amino a…

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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LADL Bridge + Target Zfp462-Klf4SE

Plasmid

#127668

Purpose

Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117

Use

CRISPR

Tags

HA

Expression

Mammalian

Promoter

EF1a and hU6

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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BRI1 BRI1_pECIA14

Plasmid

#114886

Purpose

Prey vector BRI1 BRI1_pECIA14 should be used with bait vector BRI1 BRI1_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G39400 (BRI1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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MAP4K3-GFP d344-873, K45E

Plasmid

#21735

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus, amino-acids 344…

Available Since

June 3, 2010

Availability

Academic Institutions and Nonprofits only
pPig-hEF1a-Cry2PHR-LRP6c-Puro-Blind

Plasmid

#249712

Purpose

Expresses optogenetic LRP6 in mammalian cells; no fluorescent proteins. Plasmid contains a puromycin selection marker.

Depositor

Max Wilson

Article

Rosen et al bioRxiv [Preprint]. 2025 May 23:2025.02.

Inserts

Cry2-LRP6 (CRY2, LRP6 Mustard Weed, Human)
P2A-PuroR

Expression

Mammalian

Promoter

hEF1a

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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synapsin-hIRβ-mCherry

Plasmid

#228449

Purpose

Expressing a truncated, constitutively active human insulin receptor (IRβ) with mCherry in rat primary hippocampal neurons

Depositor

Olivier Thibault

Article

Frazier et al Neurochem Res. 2019 Jan;44(1):269-280. d

Insert

human Insulin Receptor beta subunit (INSR Human)

Use

AAV

Promoter

neuron-specific synapsin promote

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-Hb9-CD14

Plasmid

#204344

Purpose

Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.

Depositor

Ivo Lieberam

Article

Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

Insert

human CD14 (CD14 Human)

Expression

Mammalian

Promoter

mouse Hb9 (Mnx1) 9kb promoter fragment

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_DCAF11:1-546

Plasmid

#210907

Purpose

Insect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

R207H natural variant (VAR_020121)

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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