We narrowed to 1,479 results for: pbluescript

1. pMK322 (TOP2A-mAC (Hygro))

   Plasmid

   #140653

   Purpose

   TOP2A tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-f1(-)-ACTB-mEGFP

   Plasmid

   #115917

   Purpose

   donor to insert EGFP at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-mEGFP
   

   Use

   Donor template

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRepair-mScI-CTNNB1

   Plasmid

   #153431

   Purpose

   Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
   

   Use

   CRISPR; Hdr donor template

   Tags

   mScarlet-i

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-f1(-)-GAPDH-IRES-EGFP

   Plasmid

   #115918

   Purpose

   donor to insert IRES-EGFP at the GAPDH locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   IRES-EGFP
   

   Use

   Donor template

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-f1(-)-TOMM20-mEGFP

   Plasmid

   #115923

   Purpose

   donor to insert EGFP at the TOMM20 locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   TOMM20-mEGFP
   

   Use

   Donor template

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. tet H-ras val12

   Plasmid

   #8900

   Depositor

   Lynda Chin

   Article

   Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

   Insert

   ras val12 (HRAS Human)
   

   Use

   Tags

   Tet-op promoter

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   Jan. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

7. INCTbiosyn-pAct-AtINTphiC31

   Plasmid

   #127535

   Purpose

   Plasmid encodes A. thaliana codon optimized Integrase phiC31.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase phiC31 coding sequence codon optimized for A. thaliana expression.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LB335 (Bcl-2 from ATG to -1281)

   Plasmid

   #15382

   Depositor

   Linda Boxer

   Article

   Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508

   Insert

   B-cell lymphoma/leukemia-2 (BCL2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Bacterial

   Mutation

   Bcl-2 fragment from ATG to -1281 with P2

   Promoter

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pBS-mHopX

   Plasmid

   #16425

   Depositor

   Jonathan Epstein

   Article

   Chen et al Cell. 2002 Sep 20. 110(6):713-23.

   Insert

   Hopx (hop Mouse)
   

   Use

   In situ template

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. INCTbiosyn-pAct-AtINTBxb1

    Plasmid

    #127536

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSK+ mBMPR-1B DN

    Plasmid

    #49530

    Purpose

    cloning vector for dominant negative mouse BMP receptor 1B

    Depositor

    Lee Niswander, Peter ten Dijke

    Article

    Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

    Insert

    BMPR-1B DN (Bmpr1b Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    dominant negative mutation (K231R); also L2V

    Promoter

    T3

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pRepair-SYFP2-CTNNB1

    Plasmid

    #153432

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    SYFP2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. DICER KO vector

    Plasmid

    #16656

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

    Insert

    Dicer knock-out (DICER1 Human)
    

    Use

    Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pBS-f1(-)-SEC61B-mEGFP

    Plasmid

    #115921

    Purpose

    donor to insert mEGFP at the SEC61B locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SEC61B-mEGFP
    

    Use

    Donor template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid

    #70666

    Purpose

    Reduced the expression of gamma-tubulin in murine cells

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    

    Use

    U6 promoter

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-f1(-)-GAPDH-IRES-dTomato

    Plasmid

    #115919

    Purpose

    donor to insert IRES-dTomato at the GAPDH locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    IRES-dTomato
    

    Use

    Donor template

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. b2AR->M71-IRES-tauGFP-ACNF TV

    Plasmid

    #105198

    Purpose

    targeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.

    Depositor

    Peter Mombaerts

    Article

    Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

    Insert

    b2AR->M71-IRES-tauGFP-ACNF (Adrb2, Olfr151 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMADM-alpha

    Plasmid

    #36890

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Neo
    ATG (T)
    GFP
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, deleted…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2-7xTcf-NLS-ONL-CP

    Plasmid

    #65716

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2-7xTcf-NLS-YNL-CP

    Plasmid

    #65714

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge