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Showing: 1401 - 1420 of 13298 results

  2. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

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  4. EB3-3xmScarletI-N1

    Plasmid
    #112928
    Purpose
    In vivo visualization of microtuble plus ends (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    EB3
    Use
    Tags
    3x mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112928-plasmid-map-sequence-id-223510

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  5. mScarlet-I-PXN

    Plasmid
    #112956
    Purpose
    In vivo visualization of focal adhesions (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    PXN
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112956-plasmid-map-sequence-id-222564

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  7. pBlueScript-humanAPOBEC1

    Plasmid
    #134606
    Purpose
    Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    humanAPOBEC1 (APOBEC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Beta actin
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134606-plasmid-map-sequence-id-258758

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  8. ITPKA-mScarlet-I

    Plasmid
    #98829
    Purpose
    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    ITPKA
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98829-plasmid-map-sequence-id-189652

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  10. pGEM-3XP3.RFP-tan

    Plasmid
    #83474
    Purpose
    RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.
    Depositor
    Patricia Wittkopp
    Article
    Lamb et al Fly (Austin). 2016 Aug 5:1-12.
    Insert
    3XP3.RFP-tan
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    3XP3 eye-expression Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83474-plasmid-map-sequence-id-159168

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  11. mScarlet-I-UtrCH

    Plasmid
    #98823
    Purpose
    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    UtrCH
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    98823-plasmid-map-sequence-id-190901

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  12. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

    Plasmid
    #122964
    Purpose
    Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.
    Depositor
    Ken-Ichi Takemaru, FENG-QIAN Li
    Insert
    human Chibby1 (CBY1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mutation
    Promoter
    FoxJ1 and human FoxJ1
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122964-plasmid-map-sequence-id-291837

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  13. pAC-crRNA-Cm

    Plasmid
    #158711
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    eforCP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158711-plasmid-map-sequence-id-325466

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  14. mNeonGreen-UtrCH

    Plasmid
    #98879
    Purpose
    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    UtrCH
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    *(see below)
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98879-plasmid-map-sequence-id-191167

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  19. pCoofy48

    Plasmid
    #55186
    Purpose
    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55186-plasmid-map-sequence-id-464535

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Showing: 1401 - 1420 of 13298 results