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pcDNA3.2/V5 hsa-mir-455

Plasmid

#26319

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-455 (MIR455 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
HOXB7 (human) HIS-tag pET

Plasmid

#8538

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

His and T7

Expression

Bacterial

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-499

Plasmid

#26320

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-499 (MIR499A Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-208a

Plasmid

#26331

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-208a (Mir208a Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

Plasmid

#159658

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

Plasmid

#159657

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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L309-myc-seipin-CT

Plasmid

#64063

Purpose

myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

Insert

bscl2 (Bscl2 Mouse)

Use

Lentiviral

Tags

myc epitope

Expression

Mammalian

Mutation

Contains Arg326 to Ser443

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
HOXB5 (human) pBluescript

Plasmid

#8531

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Expression

Bacterial

Mutation

makes protein in vitro transcription/translation

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pGEX6P1‐Afu rnhB

Plasmid

#108694

Purpose

For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Insert

rnhB (AF_RS03165 Archaeoglobus fulgidus)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAG415-ADH1p-HA-rap1-K9R

Plasmid

#84531

Purpose

This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

HA

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

ADH1

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K462-463-651R

Plasmid

#84564

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K462-463-651R

Promoter

none

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K43-117-118R

Plasmid

#84565

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K43-117-118R

Promoter

none

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-220a

Plasmid

#26314

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-220a (MIR220A Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pLVX Neo TagBFP-LC3B

Plasmid

#250866

Purpose

Stable lentiviral expression of TagBFP-LC3B

Depositor

Thomas Melia

Article

Fuller et al eLife 2025 14:RP107316

Insert

LC3B (MAP1LC3B Human)

Use

Lentiviral

Tags

TagBFP

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-BFP-P2A-otMx1

Plasmid

#224553

Purpose

Expresses Oncorhynchus tshawytscha mx1 protein and BFP

Depositor

Bertrand Collet

Article

Chaumont et al Fish Shellfish Immunol. 2026 Apr;171:111

Insert

BFP-P2A-otMx1 (LOC112247236 Oncorhynchus tshawytscha)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-BFP-P2A-otMx2

Plasmid

#224554

Purpose

Expresses Oncorhynchus tshawytscha mx2 protein and BFP

Depositor

Bertrand Collet

Article

Chaumont et al Fish Shellfish Immunol. 2026 Apr;171:111

Insert

BFP-P2A-otMx2 (LOC121839060 Oncorhynchus tshawytscha)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-BFP-P2A-otMx3

Plasmid

#224555

Purpose

Expresses Oncorhynchus tshawytscha mx3 protein and BFP

Depositor

Bertrand Collet

Article

Chaumont et al Fish Shellfish Immunol. 2026 Apr;171:111

Insert

BFP-P2A-otMx3 (LOC112247235 Oncorhynchus tshawytscha)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pYihI-N-term K-R

Plasmid

#233091

Purpose

Expression of GST-YihI with N-term lysines (K) mutated to arginine (R)

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

YihI with N-term lysines (K) mutated to arginine (R)

Tags

GST

Expression

Bacterial

Mutation

N-terminal lysine residues mutated to arginine

Available Since

May 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBa.GFP-KIF13AS1371D

Plasmid

#224417

Purpose

Mammalian expression of KIF 13a S1371D

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371D (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S1371D

Promoter

Chicken Beta actin

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AS1371A

Plasmid

#224409

Purpose

Mammalian expression of KIF 13a S1371A

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371A (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S1371A

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AWLDLE

Plasmid

#224410

Purpose

Mammalian expression of KIF 13a WLDLE

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a WLDLE (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WLDLE

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT57

Plasmid

#218728

Purpose

Expresses N-terminally EGFP-tagged IFT57 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Funabashi et al J Cell Biol. 2018 Aug 6;217(8):2867-2876

Insert

IFT57 (IFT57 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-UDP1.0-WPRE

Plasmid

#220849

Purpose

AAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)

Depositor

Yulong Li

Article

Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

Insert

UDP1.0

Use

AAV

Promoter

human Synapsin (hSyn)

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-BirA-Linker-Myc-mRap1

Plasmid

#184644

Purpose

Retroviral expression of BirA-mRap1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

BirA-rap1

Use

Retroviral

Tags

BirA FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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puc19_N_tag

Plasmid

#186650

Purpose

Empty donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

N-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

Plasmid

#184645

Purpose

Retroviral expression of BirA Rap1 I312R

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

BirA-rap1-I312R

Use

Retroviral

Tags

BirA FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_164 SpCas9 R1333A/R1335A

Plasmid

#179528

Purpose

For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 R1333A/R1335A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginines 1333 and 1335 to alanine

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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