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pLM-AMA15.0 Cas9

Plasmid

#138944

Purpose

Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers

Depositor

Arnold Driessen

Article

Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

Insert

p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers

Use

CRISPR and Synthetic Biology; Ama1 autonomously r…

Tags

NLS

Expression

Mutation

Promoter

p40S (AN0465) Aspergillus nidulans

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLM-AMA18.0 dCas9-VPR

Plasmid

#138945

Purpose

Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers

Depositor

Arnold Driessen

Article

Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

Insert

p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers

Use

CRISPR and Synthetic Biology; Ama1 autonomously r…

Tags

NLS

Expression

Mutation

Promoter

p40S (AN0465) Aspergillus nidulans

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HF-BE3

Plasmid

#87439

Purpose

Expresses HF-BE3 with C-terminal NLS in mammalian cells

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Use

Tags

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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Target-AID (pRZ762)

Plasmid

#131300

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

Plasmid

#201684

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1 (V106W)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U1a

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3H-BE3

Plasmid

#113416

Purpose

Expresses hA3H-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3H-BE3 (APOBEC3H S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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HA-KIF5B*-strep

Plasmid

#120170

Purpose

Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Use

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4GamRA-P2A-Puro

Plasmid

#112673

Purpose

Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4GamRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-SI-121

Plasmid

#131126

Purpose

A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

mutant EGFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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BE3-P2A-EGFP (pJUL977)

Plasmid

#123612

Purpose

CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-BE3RA-PGK-Puro

Plasmid

#110846

Purpose

Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

TRE3G

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMonAID_nCas9-PmCDA_Hyg_ALS

Plasmid

#91693

Purpose

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

2x35S

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3-Y130F

Plasmid

#113428

Purpose

Expresses hA3A-BE3-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE-YEE

Plasmid

#107687

Purpose

Expresses dLbCpf1-BE-YEE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE-YEE

Use

Tags

Expression

Mammalian

Mutation

APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

Promoter

CMV

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLL4 sgRNA(MS2)-MS2-hA3a

Plasmid

#112130

Purpose

empty sgRNA cloning vector with MS2-humanA3a

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a (APOBEC3A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6,CBh

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-BE3.9max N-terminal

Plasmid

#137171

Purpose

Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-BE3.9max_N-terminal

Use

Tags

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-ABEmax N-terminal

Plasmid

#137173

Purpose

Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-ABEmax_N-terminal

Use

Tags

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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mCh-KIF1A*-strep

Plasmid

#120165

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 1A (Kif1a Mouse)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF1A: 1-405 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-HA

Plasmid

#120171

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Use

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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