We narrowed to 2,303 results for: dap

1. pBa.GFP-KIF3A tail 419-702

   Plasmid

   #193923

   Purpose

   Mammalian expression of GFP-tagged Kif 3A tail 419-702

   Depositor

   Marvin Bentley

   Article

   Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

   Insert

   Kif 3A (KIF3A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR2_+10D+7U

   Plasmid

   #149393

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   7bp inserted upstream, and 10bp inserted downstre…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR2_+10D+10U

   Plasmid

   #149394

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   10bp inserted upstream and downstream of the IHFp…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR2_IHF_Mut

   Plasmid

   #149396

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   IHFprox site is mutated at key positions needed f…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFL GST-UBXN2A

   Plasmid

   #169016

   Purpose

   Expresses GST-UBXN2A (human) in insect cells

   Depositor

   Hemmo Meyer

   Article

   Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

   Insert

   UBXN2A (UBXN2A Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMKO.1-shNuf2

   Plasmid

   #160961

   Purpose

   Nuf2 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nuf2 shRNA (Nuf2 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EcRfaHKOW-L1L2NusG

   Plasmid

   #113126

   Purpose

   Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   RfaH (rfaH Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-107, replaced L1 and L2 loops …

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28P_dCrk

   Plasmid

   #131145

   Purpose

   Expresses 6XHis::dCrk (based on crk isoform RA).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-biotin-myc- mCE

   Plasmid

   #82475

   Purpose

   Expresses mRNA capping enzyme containing N terminal biotin and myc tag

   Depositor

   Daniel Schoenberg

   Article

   Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

   Insert

   Biotin (Rngtt Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF-HA-TSAd-Y89XF

    Plasmid

    #84559

    Purpose

    mammalian expression of TSAd-Y89XF

    Depositor

    Anne Spurkland

    Article

    Granum et al J Biol Chem. 2008 Aug 8;283(32):21909-19

    Insert

    TSAd (SH2D2A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y279F, Y290F, Y305F

    Promoter

    EF1a

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pET28a-Hook3 Q147A, M151A, I154A

    Plasmid

    #74616

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Q147A, M151A, I154A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Hook3 D102A, E108A

    Plasmid

    #74618

    Purpose

    Expresses a double mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-Hook3 N68A, W69A, K77A

    Plasmid

    #74619

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid

    #227332

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. hLRP1-pEZT-BM

    Plasmid

    #177985

    Purpose

    Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

    Depositor

    Lora Hooper

    Article

    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

    Insert

    LDL receptor related protein 1 (LRP1 Human)
    

    Use

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MYC-hTRAK2

    Plasmid

    #225142

    Purpose

    Expresses MYC-tagged human TRAK2

    Depositor

    Thomas Schwarz

    Article

    Davis et al Life Sci Alliance. 2022 Oct 27;6(1):e202

    Insert

    TRAK2 (TRAK2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMRX-IPU-GFP-MYH9

    Plasmid

    #168273

    Purpose

    Expresses MYH9 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    MYH9 (MYH9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. qTAG-C-moxGFP-Puro-H2BC11

    Plasmid

    #207758

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid

    #227275

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-LAMP1

    Plasmid

    #207787

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge