We narrowed to 9,995 results for: pET

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pET-28a RUVBL2 Delete

Plasmid

#159143

Purpose

Expression and protien purification of a mutated RUVBL2

Depositor

Eric Erquan Zhang

Publication

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL2 (RUVBL2 Human)

Expression

Bacterial

Mutation

amino acid 2-456, Thr127-Glu233 mutated to GPPG

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PET.SUMO eIF4G1 +MIC 1-200

Plasmid

#158792

Purpose

Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

Depositor

Benjamin Blencowe

Publication

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon) codon optimized

Tags

6xHIS and SUMO

Expression

Bacterial

Mutation

amino acids 1-200

Promoter

T7

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK jannaschii

Plasmid

#108948

Purpose

heterologous expression of MVK of M. jannaschii in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET-CH2 (1192-1318)

Plasmid

#99338

Purpose

Bacterial expression of mouse CBP CH2 domain

Depositor

Peter Wright

Publication

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

CBP CH2 (1192-1318) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL4-V5-His6

Plasmid

#67419

Purpose

Expression of hArl4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4C-V5-His6

Plasmid

#67423

Purpose

Expression of hArl4c with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Publication

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Rv2002 M3/pET-28b(+)N-tag

Plasmid

#13057

Depositor

SeWon Suh

Publication

Yang et al Proc Natl Acad Sci U S A. 2003 Jan 21. 1

Insert

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

Tags

His

Expression

Bacterial

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET-28b(+)-DR RecR(34-220)

Plasmid

#12618

Depositor

SeWon Suh

Publication

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR(34-220)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-33

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET-21a(+) SurE D9A

Plasmid

#12686

Depositor

SeWon Suh

Publication

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE

Tags

His

Expression

Bacterial

Mutation

changed Aspartate 9 to Alanine

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pet 3d alpha tentacle delete CP

Plasmid

#13299

Depositor

John Cooper

Publication

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits (CAPZB Chicken)

Expression

Bacterial

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pet 3d beta tentacle delete CP

Plasmid

#13300

Depositor

John Cooper

Publication

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 1 (deletion 28 amino acids ) (CAPZB Chicken)

Expression

Bacterial

Mutation

CP alpha subunit is intact with a mutated beta C-…

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pet 3d double tentacle delete CP

Plasmid

#13301

Depositor

John Cooper

Publication

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids)

Expression

Bacterial

Mutation

Capping protein alpha 1 (with 28 amino acids dele…

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Publication

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PIA900

Plasmid

#104401

Purpose

Polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Publication

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA, B, C, and Z

Use

E. coli expression vector

Tags

encodes a C-terminally His-tagged rpoC which can …

Expression

Bacterial

Promoter

T7

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBz172_CsoS2_N1_GFP

Plasmid

#140852

Purpose

His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 1 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBz175_CsoS2_N4_GFP

Plasmid

#140855

Purpose

His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 4 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz173_CsoS2_N2_GFP

Plasmid

#140853

Purpose

His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 2 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz47_polyPro_CsoS2_Npeptide

Plasmid

#140845

Purpose

Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 N-peptide

Tags

Hexahistidine tag and Strep-tag II

Expression

Bacterial

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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