Skip to main content
Addgene
Showing: 1401 - 1420 of 14547 results

  1. pTS2343_Tier3(SB)-YPet_Blast

    Plasmid
    #169641
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven YPet and BlastR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169641-plasmid-map-sequence-id-366220

    Enlarge

  2. pET24TEV-tSHIP1

    Plasmid
    #183770
    Purpose
    Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    William Kerr
    Article
    Sudan et al Front Immunol. 2022 May 4;13:830961. doi
    Insert
    SHIP1 (INPP5D Human)
    Use
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa1 to aa883 only
    Promoter
    T7 lac
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183770-plasmid-map-sequence-id-365832

    Enlarge

  3. pET24TEV-tSHIP2

    Plasmid
    #183771
    Purpose
    Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    William Kerr
    Article
    Sudan et al Front Immunol. 2022 May 4;13:830961. doi
    Insert
    SHIP2 (INPPL1 Human)
    Use
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa18 to aa985 only
    Promoter
    T7 lac
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183771-plasmid-map-sequence-id-362691

    Enlarge

  10. pET28a(+)-HisTim12_CtoA

    Plasmid
    #170281
    Purpose
    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2
    Insert
    yeast Tim12 (TIM12 Budding Yeast)
    Use
    Tags
    TEV-cleavable N-terminal His tag (on backbone)
    Expression
    Bacterial
    Mutation
    mutated two non-conserved Cys to Ala
    Promoter
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170281-plasmid-map-sequence-id-334955

    Enlarge

  11. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Use
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Promoter
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

    Enlarge

  12. pET11a-N-DiB-RM

    Plasmid
    #168476
    Purpose
    N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168476-plasmid-map-sequence-id-332734

    Enlarge

  13. pET11a-Z-N-DiB-RM

    Plasmid
    #168475
    Purpose
    N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168475-plasmid-map-sequence-id-332732

    Enlarge

  14. pET11a-Z-N-DiB2

    Plasmid
    #168473
    Purpose
    N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10
    Insert
    N-fragment of DiB2 + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168473-plasmid-map-sequence-id-332731

    Enlarge

  15. pET11a-N-DiB2

    Plasmid
    #168472
    Purpose
    N-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10
    Insert
    N-fragment of DiB2
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168472-plasmid-map-sequence-id-332730

    Enlarge

  16. pET11a-Z-N-wtBlc

    Plasmid
    #168258
    Purpose
    N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    N-fragment of wtBlc + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168258-plasmid-map-sequence-id-332729

    Enlarge

Showing: 1401 - 1420 of 14547 results