We narrowed to 6,528 results for: sas

1. Rlucrarr3-Flash3

   Plasmid

   #74131

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

2. Rlucrarr3-Flash2

   Plasmid

   #74130

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Available Since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP

   Plasmid

   #138334

   Purpose

   Expresses APOB-Linker-eGFP

   Depositor

   Mahmood Hussain

   Article

   Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

   Insert

   5'UTR-ApoB48-Linker-eGFP (APOB Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-U6-pegSERPINA1-U6-Nicking-PE2-N

   Plasmid

   #164907

   Purpose

   Express a pegRNA targeting SERPINA1, and a nicking sgRNA and the N-terminal split-intein fragment of PE2

   Depositor

   Wen Xue

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   pegSERPINA1, Nicking sgRNA, PE2-N
   

   Use

   AAV

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. P25-pZE-SUMO-PbTTA

   Plasmid

   #204634

   Purpose

   ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

   Depositor

   Aditya Kunjapur

   Article

   Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

   Insert

   His-SUMO-TEV-PbTTA
   

   Use

   Synthetic Biology

   Tags

   His Tag; SUMO Tag; TEV Protease Site

   Expression

   Bacterial

   Promoter

   PLtetO-1 promoter

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p026 pCS2-EnR

   Plasmid

   #11028

   Depositor

   Ramesh Shivdasani

   Article

   Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

   Insert

   engrailed (en Fly)
   

   Use

   Xenopus, mammalian, avian, and zebrafish

   Mutation

   Engrailed repressor domain (bases encoding amino …

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Tag4A-MITF-M (wt)

   Plasmid

   #31151

   Depositor

   Yardena Samuels

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   MITF-M (MITF Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   WT

   Available Since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSMP-EZH2_1

   Plasmid

   #36387

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   EZH2 (EZH2 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR-cBEST-v2-PermE

   Plasmid

   #209446

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PtipA; sgRNA expression from ermE* promoter

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-21a_FnCpf1_2xNLS_protein_expression

    Plasmid

    #124548

    Purpose

    Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnCpf1_2xNLS
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5 FLAG human DEPTOR

    Plasmid

    #21334

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR (DEPTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N204S and S389N mutations (numbering according to…

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221-hSOX10

    Plasmid

    #24749

    Depositor

    William Pavan

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    (sex determining region Y)-box 10 (SOX10 Human)
    

    Use

    Gateway donor vector

    Mutation

    wildtype sequence

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pLYS1-MICU1-Flag

    Plasmid

    #50058

    Purpose

    Expression of human MICU1 with Flag tag in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    MICU1 (isoform 2) (MICU1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    *

    Available Since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpyCas9 PE2*

    Plasmid

    #169850

    Purpose

    Mammalian Expression, SpyCas9 prime editor, optimized NLS

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    spCas9 PE*
    

    Tags

    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWPXL-Sox4

    Plasmid

    #36984

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Sox4 (SOX4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH2685

    Plasmid

    #191372

    Purpose

    Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Tetanus Toxin Light Chain
    

    Tags

    wCherry

    Expression

    Worm

    Promoter

    Punc-4

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LUC01 (L/Sec/P)

    Plasmid

    #112144

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U)

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-TRIM28deltaLinker

    Plasmid

    #124958

    Purpose

    Mammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domains

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of linker region between CC domain and P…

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. NgOE FUNgGW

    Plasmid

    #92231

    Purpose

    Lentiviral expression of human Ng

    Depositor

    Weifeng Xu

    Article

    Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

    Insert

    Ng (NRGN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNAintron-CHIKV-Envelope protein

    Plasmid

    #215700

    Purpose

    Produces the Chikungunya virus envelope protein E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV E3-E2-6K-E1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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