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Showing: 3551 - 3600 of 4184 results

  2. ARL5:H14-K177 (2H16)

    Plasmid
    #25526
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ARL5:H14-K177 (ARL5A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids H14-K177
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. GNE

    Plasmid
    #25255
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GNE:T406-R720 (GNE Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids T406-R720
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. ZNF289

    Plasmid
    #25232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ZNF289:A3-A127 (ARFGAP2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids A3-A127
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. SETDB1

    Plasmid
    #25240
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1, SETDB1_16 (SETDB1 Human)
    Use
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. SpyTag-β-Lactamase-SpyCatcher

    Plasmid
    #52656
    Purpose
    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.
    Depositor
    Mark Howarth
    Article
    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2
    Insert
    TEM-1 β-Lactamase
    Use
    Tags
    SpyCatcher and SpyTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52656-plasmid-map-sequence-id-422741

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  21. SpyTag003-MBP

    Plasmid
    #133450
    Purpose
    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    SpyTag003-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133450-plasmid-map-sequence-id-267039

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  29. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

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  32. ITCH

    Plasmid
    #36197
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH (ITCH Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains HECT domain
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. AMPKA2

    Plasmid
    #36194
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    AMPKA2 (PRKAA2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains KINASE DOMAIN only
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  42. Fast-GCaMP-EF20

    Plasmid
    #52645
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF20
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D362N/D366N mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. Fast-GCaMP-EF16

    Plasmid
    #52642
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF16
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D431N/D433N/D435N/E442A mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  46. HTATIP (2OU2)

    Plasmid
    #33338
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HTATIP (KAT5 Human)
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. Fast-GCaMP-EF19

    Plasmid
    #52644
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF19
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T328D/T330D mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 3551 - 3600 of 4184 results