We narrowed to 7,541 results for: URE
-
Plasmid#74205PurposeRetroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codonDepositorInsertneighbor of BRCA1 gene 1 (NBR1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationChanged aa 877 to encode for premature stop codonAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Integrin beta1(1-455)-tev-BHCys
Plasmid#27315DepositorInsertintegrin beta1 (ITGB1 Human)
ExpressionMammalianMutationbeta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6Available SinceApril 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 pBabe puro
Plasmid#13604DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseRetroviralExpressionMammalianMutationcontains mature ITGA9 (aa30-1035 compared to NP_0…Available SinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)
Plasmid#213028PurposeExpresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
Tags3xFlag (ORF2p)ExpressionMammalianMutationE43S D145N double mutant EN- endonuclease catalyt…PromoterCMVAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-mtEFTu
Plasmid#30535DepositorInsertMature bovine mitochondrial elongation factor Tu
TagsHisExpressionBacterialMutationMature form amino acids 44-452. Mito import seque…Available SinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpaCas12f1-gRNA_MS13
Plasmid#190877PurposeThe plasmid of CRISPR/SpaCas12f1(Syntrophomonas palmitatica Cas12f1) for genome editing in human cellsDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-SaCas9
Plasmid#129553PurposeContains codon-optimized SaCas9 and gRNA for streptomycesDepositorInsertSaCas9
ExpressionBacterialPromoterrpsL(XC)-BbsIAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSW002
Plasmid#111248PurposeBroad host-range bacterial vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MCS Hras FI
Plasmid#90413PurposeA control of MCS+ that associated with cell plasma membrane by two hydrophobic interaction at N and C terminus of the FRET pair.DepositorInsertLck10+ Venus+mcherry + H-ras C terminus
ExpressionMammalianAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMU102
Plasmid#61805PurposeCloning and expression vector for Clostridium thermocellumDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-E112A
Plasmid#110215PurposeExpression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsFlagExpressionMammalianMutationE112A, P296L (see depositor comments below)Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA
Plasmid#64983PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-4xnrUAS
Plasmid#87120PurposeMultisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elementsDepositorInsert4xnrUAS (non repetitive)
UseGateway vectorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastbacHTB HA-human PP2A Cα D88N
Plasmid#132633Purposeexpresses HA-human PP2A Cα D88N in Hi5 insect cellDepositorInsertPP2A Cα D88N
ExpressionInsectAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pAcCas12nHS_RV6_empty
Plasmid#203811PurposePlasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.DepositorInsertAcCas12nHS_RV6
UseCRISPRPromoterCMVAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
1B-GFP1-10
Plasmid#193867PurposeE. coli expression vector for Hisx6 tagged GFP1-10.DepositorInsertsfGFP1-10
Tags6xHisExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-gTEMP/pRSET-b
Plasmid#188446PurposeB-gTEMP for bacterial expressionDepositorInsertB-gTEMP
Tags6xHis-tagExpressionBacterialAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-TT-PA-CRE
Plasmid#179584PurposeePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)DepositorInsertPA-MAG-CRE
ExpressionMammalianPromoterTREAvailable SinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS402-GPD-GFP(S65T)
Plasmid#85190PurposeIntegrating plasmid, GFP(S65T) under GPD promoterDepositorInsertEGFP
UseIntegrating, shuttle vectorExpressionYeastPromoterGPDAvailable SinceDec. 27, 2016AvailabilityAcademic Institutions and Nonprofits only