We narrowed to 2,522 results for: plenti

1. pLenti-CMV-Pink Flamindo-p2a-bPAC

   Plasmid

   #202716

   Purpose

   Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Pink Flamindo
   bPAC
   

   Use

   Lentiviral

   Tags

   10xHis and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti HRE mutant-Luc pGK Hygro

   Plasmid

   #118707

   Purpose

   reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   HREmutant-Luc
   

   Use

   Lentiviral

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-C-Myc-DDK-IRES-Puro HNRNPA1

   Plasmid

   #200727

   Purpose

   Lentiviral expression of Flag-Tagged human HNRNPA1

   Depositor

   Claudio Alarcón

   Insert

   HNRNPA1 (HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti spCas9 T2A mNeonGreen P2A puro

   Plasmid

   #122183

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9 (NEWENTRY )
   mNeonGreen
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-HF1RA-P2A-GFP-PGK-Puro

   Plasmid

   #110866

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20944

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

   Plasmid

   #122966

   Purpose

   Expresses 3XFlag-tagged human CEP164 in mammalian cells

   Depositor

   Ken-Ichi Takemaru, FENG-QIAN Li

   Insert

   CEP164 (CEP164 Human)
   

   Use

   Lentiviral

   Tags

   3XFlag

   Promoter

   EF1 alpha

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

   Plasmid

   #20294

   Purpose

   Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Serine

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

   Plasmid

   #177320

   Purpose

   Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

   Depositor

   Steven Chu, Xiaolin Nan

   Article

   Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

   Insert

   KRAS 4B (KRAS Human)
   

   Use

   Lentiviral

   Tags

   FKBP-derived dimerization domain (DD, or DmrB) an…

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   TetOn-CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

    Plasmid

    #42651

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 399 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T399I

    Promoter

    CMV/TO

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-VQR-P2A-GFP-PGK-Puro

    Plasmid

    #110861

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid

    #122200

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9
    TagRFP-T
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204188

    Purpose

    Doxycycline inducible lentiviral vector for human NOTUM overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NOTUM (NOTUM Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

    Plasmid

    #122957

    Purpose

    Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NLB (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Promoter

    CAG promoter

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. stblHIF: pLenti-PGK-stblHIF-PGK-Puro

    Plasmid

    #177202

    Purpose

    encoding stblHIF under PGK promoter

    Depositor

    Markus Covert

    Article

    Kudo et al bioRxiv (2022)

    Insert

    stblHIF
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    P402A/P577G/N813A

    Promoter

    PGK

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

    Plasmid

    #191676

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A

    Depositor

    Veerle Baekelandt

    Inserts

    ATP10B (ATP10B Human)
    TMEM30A (TMEM30A Human)
    

    Use

    Lentiviral

    Tags

    6x HIS and flag

    Mutation

    D433N

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMV Puro DEST (w118-1) eGFP

    Plasmid

    #107505

    Purpose

    Expresses eGFP, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201151

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    SadCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Mutation

    gRNA sequence: ATCAGTGATAGAGAACGTATG

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

    Plasmid

    #200631

    Purpose

    Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    iCasp9
    

    Use

    Lentiviral

    Promoter

    pTRE3G

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CMV Puro DEST (w118-1) IGF1R

    Plasmid

    #107499

    Purpose

    Expresses IGF1R, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    IGF-1R (IGF1R Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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