165,688 results

1. slc1a3b:myrGFP-P2A-H2AmCherry

   Plasmid

   #170208

   Purpose

   Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

   Depositor

   Kelly Monk

   Article

   Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

   Insert

   slc1a3b (slc1a3b Zebrafish)
   

   Use

   Zebrafish

   Tags

   myrGFP-P2A-H2AmCherry

   Promoter

   slc1a3b

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-DNA Topoisomerase 1B Vaccinia Virus

   Plasmid

   #165510

   Purpose

   Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   DNA Topoisomerase 1B Vaccinia Virus
   

   Use

   Synthetic Biology

   Available Since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. hCas9-VPR

   Plasmid

   #68497

   Purpose

   nuclease competent SP-Cas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   SP-Cas9-VPR
   

   Tags

   VPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-TLR4-YFP

   Plasmid

   #13018

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 4 (TLR4 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. ZJOM90

   Plasmid

   #133647

   Purpose

   Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pTPI1-SS-mCherry-HDEL
   

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAWP78

   Plasmid

   #61263

   Purpose

   Small IncP-based broad host range replicating vector.

   Depositor

   Mary Lidstrom

   Article

   Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-mStayGold-EFS-Puro

   Plasmid

   #227256

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30-2-GPD1

   Plasmid

   #83911

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
   

   Tags

   6x His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMG-GRIS

   Plasmid

   #225134

   Purpose

   For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Fuw-dCas9-Dnmt3a-P2A-tagBFP

    Plasmid

    #84569

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Synthetic, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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