163,000 results

1. AAVS1-Pur-CAG-EGFP

   Plasmid

   #80945

   Purpose

   human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. cfSGFP2-anti-AlfaTag nanobody

   Plasmid

   #171818

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   cfSGFP2-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-6xGLY-3xFLAG-kanMX6

   Plasmid

   #20754

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEVOL-pylT-N346A/C348A

   Plasmid

   #127411

   Purpose

   Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

   Depositor

   Wenshe Liu

   Article

   Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

   Insert

   Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N346A-C348A

   Promoter

   araBAD

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti.PGK.LifeAct-GFP.W

   Plasmid

   #51010

   Purpose

   Lentivector that expresses LifeAct-GFP from internal human PGK promoter

   Depositor

   Rusty Lansford

   Insert

   LifeAct-GFP
   

   Use

   Lentiviral

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   Promoter

   human PGK

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-MYO5C

   Plasmid

   #135404

   Purpose

   Expresses GFP-tagged human MYO5C in mammalian cells.

   Depositor

   Richard Cheney

   Article

   Jacobs et al Mol Biol Cell. 2009 Nov;20(21):4471-88.

   Insert

   MYO5C (MYO5C Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Relative to the GenBank MYO5C cDNA sequence repor…

   Promoter

   CMV

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. miABE

   Plasmid

   #205412

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D61A+E85R+H369R+S387R+S457R

   Promoter

   hU6, CAG, CMV

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag Rab12 Q101L

   Plasmid

   #86788

   Purpose

   mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

   Depositor

   Peter McPherson

   Article

   Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

   Insert

   Rab12 (RAB12 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Q101L, constitutively GTP-bound mutant

   Promoter

   CMV

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GAL4UAS-Luciferase reporter

   Plasmid

   #64125

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   GAL4UAS
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   GAL4UAS

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-DCX

    Plasmid

    #32852

    Depositor

    Joseph Gleeson

    Article

    Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

    Insert

    DCX (DCX Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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