We narrowed to 1,174 results for: Aspa

1. His6_BcLOV4_Q355N_mCherry_BamUK

   Plasmid

   #119762

   Purpose

   Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

   Depositor

   Brian Chow

   Article

   Glantz et al Methods Enzymol. 2019;622:249-270. doi:

   Insert

   BcLOV4-Q355N_mCherry
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Mutated glutamine at position 355 to asparagine

   Promoter

   T7

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest472 SHP-1 S591D

   Plasmid

   #21806

   Depositor

   Stephen Shaw

   Article

   Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

   Insert

   SHP-1 (PTPN6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Serine 591 to Aspartic Acid

   Promoter

   Available since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

3. ASPH

   Plasmid

   #39094

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ASPH (ASPH Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMT-DREDD-V5-His

   Plasmid

   #63749

   Purpose

   Expresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   Death related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
   

   Use

   Tags

   V5-6XHis

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET FLIPE-100u

   Plasmid

   #13539

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E S118W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   S118W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pfMDH_D230A

   Plasmid

   #67725

   Purpose

   Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed aspartate 230 to alanine

   Promoter

   T7

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pfMDH_N191A

   Plasmid

   #67724

   Purpose

   Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed asparagine 191 to alanine

   Promoter

   T7

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. piHGpd/G170D

   Plasmid

   #1113

   Depositor

   Susan Lindquist

   Article

   Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

   Insert

   HSP82 (HSP82 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   HSP82 with glycine 170 changed to aspartic acid; …

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pJExpress414_GmPOPB-D661A

   Plasmid

   #92236

   Purpose

   Express D661A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-D661A mutant
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Aspartate 661 to Alanine

   Promoter

   T7 promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS406 ATG9-GFP S122D

    Plasmid

    #114667

    Purpose

    Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

    Insert

    ATG9
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    changed serine 122 to aspartic acid

    Promoter

    ATG9

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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