Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 20,110 results for: IRE;

Showing: 2161 - 2190 of 20110 results

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cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

Plasmid

#135669

Purpose

Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

Depositor

Florian Karreth

Insert

sgPten

Use

Mouse Targeting

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(V303R)-IRES-puro

Plasmid

#128659

Purpose

mammalian expression of Flag-Polß(V303R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

V303R

Promoter

CMV

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

Plasmid

#173167

Purpose

Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Calcineurin Like EF-Hand Protein 1 (CHP1 Human)

Use

Retroviral

Tags

3XFLAG

Mutation

Synonymous mutations at sgRNA sites, G2A, S6A

Promoter

MMLV

Available Since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

Plasmid

#157982

Purpose

Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

Depositor

Lijia Ma

Article

Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

Insert

S. pyogenes sgRNA cassette (Tetra-8A8G)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K72A)-IRES-puro

Plasmid

#128661

Purpose

mammalian expression of Flag-Polß(K72A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K72A

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K244A)-IRES-puro

Plasmid

#128682

Purpose

mammalian expression of Flag-Polß(K244A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K244A

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R)-IRES-puro

Plasmid

#128654

Purpose

mammalian expression of Flag-Polß(L301R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K206A)-IRES-puro

Plasmid

#128678

Purpose

mammalian expression of Flag-Polß(K206A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K206A

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM-K206A)-IRES-puro

Plasmid

#128679

Purpose

mammalian expression of Flag-Polß(TM-K206A: L301R/V303R/V306R-K206A) protein with puromycin slection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R/K206A

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM-KTM)-IRES-puro

Plasmid

#128676

Purpose

mammalian expression of Flag-Polß(TM-KTM: L301R/V303R/V306R-K41R/K61R/K81R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R/-K41R/K61R/K81R

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(KTM)-IRES-Neo

Plasmid

#128670

Purpose

mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K41R/K61R/K81R

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(V306R)-IRES-puro

Plasmid

#128655

Purpose

mammalian expression of Flag-Polß(V306R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

V306R

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(KTM)-IRES-puro

Plasmid

#128672

Purpose

mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K41R/K61R/K81R

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM-KTM)-IRES-neo

Plasmid

#128675

Purpose

mammalian expression of Flag-Polß(TM-KTM: L301R/V303R/V306R-K41R/K61R/K81R) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R-K41R/K61R/K81R

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM-K244A)-IRES-puro

Plasmid

#128680

Purpose

mammalian expression of Flag-Polß(TM-K244A: L301R/V303R/V306R-K244A) portein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R/K244A

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

Plasmid

#160069

Purpose

Conditionally overexpress collybistin (CB2SH3-) and mCitrine

Depositor

Angel de Blas

Article

George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

AAV

Tags

HA- in CB2SH3

Promoter

human Synapsin promoter

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-021 IRES-D[C333G](H-I)//Esp3I

Plasmid

#130223

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

IRES-D[C333G]

Use

Synthetic Biology

Mutation

C333G

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-023 IRES-F[C336A](H-I)//Esp3I

Plasmid

#130225

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

IRES-F[C336A]

Use

Synthetic Biology

Mutation

C336A

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-018 IRES-A[G338A](H-I)//Esp3I

Plasmid

#130220

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

IRES-A[G338A]

Use

Synthetic Biology

Mutation

G338A

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-019 IRES-B[C335T](H-I)//Esp3I

Plasmid

#130221

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

IRES-B[C335T]

Use

Synthetic Biology

Mutation

C335T

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-020 IRES-C[A334G](H-I)//Esp3I

Plasmid

#130222

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

IRES-C[A334G]

Use

Synthetic Biology

Mutation

A334G

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-019 FLAG IFP1.4[1] IRES IFP1.4[2]

Plasmid

#130129

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

IFP1.4

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-020 FLAG miRFP670[1] IRES miRFP670[2]

Plasmid

#130130

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

miRFP670

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-EMMI-022 IRES-E[T337A](H-I)//Esp3I

Plasmid

#130224

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

IRES-E[T337A]

Use

Synthetic Biology

Mutation

T337A

Available Since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS

Plasmid

#131354

Purpose

Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

Caspase-1 (C285A)-NpLxIS (Casp1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Caspase-1 (C285A)

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

Plasmid

#121748

Purpose

pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

OLLAS epitope tag-IRES2-eGFP + polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FHH-NRASQ61K/T35A-IRES-Puro

Plasmid

#120571

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/Y40A-IRES-Puro

Plasmid

#120573

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FHH-NRASQ61K/E37A-IRES-Puro

Plasmid

#120572

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, E37A

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2-Cx43-M100/125/147L-siResist

Plasmid

#49856

Purpose

Encodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, and Methionine 147…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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