We narrowed to 5,173 results for: pcDNA3.1+

1. pcDNA3.1 Flag YFP hNKCC1 A675C (NT441)

   Plasmid

   #50876

   Purpose

   Expresses human NKCC1 A675C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   A675C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Flag YFP hNKCC1 I730C (NT504)

   Plasmid

   #50884

   Purpose

   Expresses human NKCC1 I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   I730C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 Flag YFP hNKCC1 A735C (NT509)

   Plasmid

   #50889

   Purpose

   Expresses human NKCC1 A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   A735C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 Flag YFP hNKCC1 S679C (NT447)

   Plasmid

   #50880

   Purpose

   Expresses human NKCC1 S679C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   S679C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 Flag YFP hNKCC1 N680C (NT468)

   Plasmid

   #50881

   Purpose

   Expresses human NKCC1 N680C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   N680C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 Flag YFP hNKCC1 W732C (NT506)

   Plasmid

   #50886

   Purpose

   Expresses human NKCC1 W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   W732C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 Flag YFP hNKCC1 L671C (NT440)

   Plasmid

   #50872

   Purpose

   Expresses human NKCC1 L671C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   L671C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 Flag YFP hNKCC1 E670C (NT473)

   Plasmid

   #50871

   Purpose

   Expresses human NKCC1 E670C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   E670C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 Flag YFP hNKCC1 C820S (NT573)

   Plasmid

   #49075

   Purpose

   Expresses human NKCC1 C820S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   C820S in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Flag YFP hNKCC1 C1052S (NT674)

    Plasmid

    #49076

    Purpose

    Expresses human NKCC1 C1052S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C1052S in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 Flag YFP hNKCC1 C791S (NT572)

    Plasmid

    #49074

    Purpose

    Expresses human NKCC1 C791S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C791S in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 Flag YFP hNKCC1 C543M (NT360)

    Plasmid

    #49068

    Purpose

    Expresses human NKCC1 C543M mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C543M in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 Flag YFP hNKCC1 C630S (NT400)

    Plasmid

    #49072

    Purpose

    Expresses human NKCC1 C630S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C630S in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 Flag YFP hNKCC1 C630A (NT399)

    Plasmid

    #49071

    Purpose

    Expresses human NKCC1 C630A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C630A in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868)

    Plasmid

    #49079

    Purpose

    Expresses human NKCC1 mutant lacking cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C295A, C543M, C563S,C568S,C577S,C582S, C630S, C72…

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859)

    Plasmid

    #49078

    Purpose

    Expresses human NKCC1 mutant lacking cysteine residues within transmembrane domains and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic,containing convenient restriction sites

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C295A, C543M, C630S, C723S, C724V in hNKCC1 in NT…

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 Flag YFP hNKCC1 C295A (NT104)

    Plasmid

    #49067

    Purpose

    Expresses human NKCC1 C295A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C295A in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 Flag YFP hNKCC1 C295S (NT103)

    Plasmid

    #49066

    Purpose

    Expresses human NKCC1 C295S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C295S in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1

    Plasmid

    #42185

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    G protein Gamma 3(F70A, F71A) (Gng3 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    F70A, F71A

    Promoter

    cmv

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

20. EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1

    Plasmid

    #42188

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    Gamma 3(K68A,K69A) (Gng3 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K68A,K69A

    Promoter

    cmv

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3.1_HA-p115-TEV-cleaved #957#382

    Plasmid

    #42107

    Depositor

    Ayano Satoh

    Article

    Koreishi et al Antibody Technology Journal. 2011;1:5-11

    Insert

    p115
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted 886 to end and added ENLYFQ

    Promoter

    CMV

    Available since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

    Plasmid

    #138198

    Purpose

    FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and cpV[E172]

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

    Plasmid

    #168097

    Purpose

    HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nGFP-(EAAAK)4-OGA(544-706)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

    Plasmid

    #79620

    Purpose

    Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1 and UGI

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES

    Plasmid

    #69909

    Purpose

    Expresses a GFP circular RNA containing the sense EMCV IRES in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1, GFP (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid

    #201819

    Purpose

    A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    hChR2(H134R)-V5-ER-LBD (ChRERa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pOTTC1751

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

    Plasmid

    #168102

    Purpose

    HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nEPEA-(EAAAK)3-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)

    Plasmid

    #60606

    Purpose

    Mammalian Display vector

    Depositor

    Wayne Marasco

    Article

    Taube et al PLoS One. 2008 Sep 11;3(9):e3181. doi: 1

    Insert

    PS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3.1(+) CircRNA Mini Vector-ZKSCAN1 nt 548-1047

    Plasmid

    #60649

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. (424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)

    Plasmid

    #168191

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nLag6-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge