Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,366 results for: pcDNA3.1+

Showing: 2161 - 2190 of 5366 results

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pcDNA3.1-SLC25A46[R257Q]-P2A-Electra1 (pAH11)

Plasmid

#227616

Purpose

Overexpression of SLC25A46 with R257Q mutation in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

R257Q

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[R340C]-P2A-Electra1 (pAH12)

Plasmid

#227617

Purpose

Overexpression of SLC25A46 with R340C mutation in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

R340C

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13)

Plasmid

#227618

Purpose

Overexpression of SLC25A46 with I349D mutation in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

I349D

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

Plasmid

#187725

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-380

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

Plasmid

#187726

Purpose

Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-491

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A

Plasmid

#215900

Purpose

Expression of TNRC6A-CIM1 domain with S1332A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1332A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A

Plasmid

#215901

Purpose

Expression of TNRC6A-CIM1 domain with S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A

Plasmid

#215903

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1616A/S1691A

Plasmid

#215904

Purpose

Expression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1616A, S1691A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C, K96H, P29S)

Plasmid

#224282

Purpose

Cell transfection and expression of Rac1 (G12C, K96H, P29S)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

G12C, K96H, P29S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

Plasmid

#218461

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )

Expression

Mammalian

Mutation

Thr1273AGlu substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

Plasmid

#218462

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )

Expression

Mammalian

Mutation

Thr1273Asp substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala

Plasmid

#218463

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )

Expression

Mammalian

Mutation

Lys1269Ala and His1271Ala substitutions

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔST-FLAG

Plasmid

#205623

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted ST domain

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔIDR3-FLAG

Plasmid

#205624

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted IDR3 domain

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔFA-FLAG

Plasmid

#205625

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted FA domain

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔDH-FLAG

Plasmid

#205629

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted DH domain

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-Y177F-FLAG

Plasmid

#205627

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

Y177F

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔAB-FLAG

Plasmid

#205628

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted AB domain

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del18-197)-FLAG

Plasmid

#204539

Purpose

Mammalian expression of human CALR ins5 (del18-197)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del18-197)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del18-197)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del198-430)-FLAG

Plasmid

#204538

Purpose

Mammalian expression of human CALR ins5 (del198-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del198-430)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del198-430)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del367-430)-FLAG

Plasmid

#204536

Purpose

Mammalian expression of human CALR ins5 (del367-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del367-430)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del367-430)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del309-430)-FLAG

Plasmid

#204537

Purpose

Mammalian expression of human CALR ins5 (del309-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del309-430)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del309-430)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del1-308)-FLAG

Plasmid

#204535

Purpose

Mammalian expression of human CALR ins5 (del1-308)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del1-308)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del1-308)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/CALR ins5 (del198-308)-FLAG

Plasmid

#204540

Purpose

Mammalian expression of human CALR ins5 (del198-308)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del198-308)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del198-308)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/CALR ins5 (del1-197)-FLAG

Plasmid

#204534

Purpose

Mammalian expression of human CALR ins5 (del1-197)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del1-197)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del1-197)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-BCR-ABL-p210-ΔTK-FLAG

Plasmid

#205630

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted tyrosine kinase (TK) domain

Promoter

CMV

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNot4_275-575-del377-402_AG

Plasmid

#148753

Purpose

Mammalian Expression of HsNot4_275-575-del377-402

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del377-402 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575-del377-424_AG

Plasmid

#148754

Purpose

Mammalian Expression of HsNot4_275-575-del377-424

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del377-424 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNot4_275-575-del400-428_AG

Plasmid

#148755

Purpose

Mammalian Expression of HsNot4_275-575-del400-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del400-428 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2161 - 2190 of 5366 results

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