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ATX3-JD

Plasmid

#184247

Purpose

Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)

Depositor

Sandra Macedo Ribeiro

Article

Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

Insert

Ataxin-3 Josephin domain (ATXN3 Human)

Tags

6x His-Tag and TEV cleavage site

Expression

Bacterial

Mutation

Premature stop codon V183X

Promoter

T7 promoter

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-hcGAS

Plasmid

#108674

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (CGAS Human)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2 SH2-SH3

Plasmid

#46442

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 (GRB2 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 1-217

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHIP1-SH2

Plasmid

#46492

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHIP1 (INPP5D Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-118

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-RAB11a KI

Plasmid

#131499

Purpose

Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rab11a, Rab11a Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2-SH2

Plasmid

#46440

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 (GRB2 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(NC)-SH2

Plasmid

#46471

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(NC) (PLCG1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 542-759

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Abl-SH2

Plasmid

#46402

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Abl (ABL1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 120-225

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX CblB-SH2

Plasmid

#46413

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

CblB (CBLB Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 42-359

Promoter

Tac

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Btk-SH2

Plasmid

#46412

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Btk (BTK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 271-385

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Blnk-SH2

Plasmid

#46408

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Blnk (BLNK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 333-456

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Grap2-SH2

Plasmid

#46435

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grap2 (GRAP2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 52-159

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Fgr-SH2

Plasmid

#46427

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Fgr (FGR Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 137-245

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Jak3-SH2

Plasmid

#46449

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Jak3 (JAK3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 366-494

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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p53 S23A targeting vector

Plasmid

#12167

Depositor

Tyler Jacks

Article

MacPherson et al EMBO J. 2004 Sep 15. 23(18):3689-99.

Insert

p53 (Trp53 Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

Ser23 mutation (S23A) in exon 2 and puromycin gen…

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Socs2-SH2

Plasmid

#46505

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs2 (SOCS2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 28-162

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Grb10-PIR-SH2

Plasmid

#46436

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb10 (GRB10 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 355-548

Promoter

Tac

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Matk-SH2

Plasmid

#46453

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Matk (MATK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 111-222

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMAPLe4 PCMTD1

Plasmid

#246503

Purpose

Recombinant protein expression of PCMTD1

Depositor

Steven Clarke

Article

Warmack et al Biochemistry. 2022 May 17;61(10):879-894

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Residue 312 is Isoleucine in this plasmid but occ…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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