We narrowed to 11,220 results for: NSI

1. pJP-HBB-nLuc

   Plasmid

   #175431

   Purpose

   Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nLUC plus 5' HBB leader
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTracer_Cr-TRP1

   Plasmid

   #64881

   Purpose

   Expresses CrTRP1 in mammalian cells

   Depositor

   Sebastián Brauchi

   Article

   Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

   Insert

   Transient receptor potential 1
   

   Use

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40

   Plasmid

   #100838

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1 and AAV9

   Insert

   GCaMP6m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CAG.GCaMP6m.WPRE.SV40

   Plasmid

   #100840

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1

   Insert

   GCaMP6m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CAG.Flex.GCaMP6m.WPRE.SV40

   Plasmid

   #100839

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cytosolic Fyn FRET Biosensor

   Plasmid

   #124654

   Purpose

   FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

   Insert

   Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1_miRFP703

   Plasmid

   #183759

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   miRFP703
   

   Use

   Tags

   Lck lipidation signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-mCherry-attP(C31)-*BleoR

   Plasmid

   #183752

   Purpose

   phiC31 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   FRT-mCherry-attP-BleoR-F3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS::AtHAP2-V5

   Plasmid

   #132958

   Purpose

   Express AtHAP2 full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

   Insert

   AtHAP2 (GCS1, HAP2 Mustard Weed)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX C_K nsp1 CoV2

    Plasmid

    #175516

    Purpose

    Expresses C_K for bioconjugation on nsp1

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    C_K nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSB161 - pL2_pSB90_2x35S::GUS::tMAS

    Plasmid

    #123197

    Purpose

    binary plant vector for transient expression of GUS (with introns)

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::GUS::tMAS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1-ASAP2f

    Plasmid

    #183757

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    ASAP2f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX nsp1 R99A CoV2

    Plasmid

    #175515

    Purpose

    For protein expression of R99A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    R99A nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO-BcRF1-2xStrep

    Plasmid

    #138436

    Purpose

    Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1 (BcRF1 EBV (HHV-4))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. b2-TS3

    Plasmid

    #103877

    Purpose

    full-length human b2 with tension sensor at b2_733

    Depositor

    Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    full-length human b2 with tension sensor at b2_733 (ITGB2 Human)
    

    Use

    Tags

    tension sensor module

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO-UL87-2xStrep

    Plasmid

    #138434

    Purpose

    Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    UL87 (UL87 HCMV (HHV-5))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1_jRCaMP1b

    Plasmid

    #183758

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    jRCaMP1b
    

    Use

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgCTRL-hygro

    Plasmid

    #199639

    Purpose

    sgRNA control; induces CAS9 cutting in an intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1 AT1AR-TSTS/4A

    Plasmid

    #45634

    Depositor

    Robert Lefkowitz

    Article

    Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

    Insert

    AT1AR-TSTS/A (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Thr332, Ser335, Thr336, and Ser338 all changed to…

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pMAL-c2X-ORF24-NTD

    Plasmid

    #138465

    Purpose

    Expresses MBP-tagged ORF24-NTD in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Amino acids 1-201

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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