We narrowed to 10,918 results for: NSI

1. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

   Plasmid

   #153446

   Purpose

   E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.CohE
   

   Use

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6H-SUMO3-ORF24-NTD-Strep

   Plasmid

   #138467

   Purpose

   Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   SUMO3 and Strep-Tag II

   Expression

   Bacterial

   Mutation

   Amino acids 2-201

   Promoter

   T7

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-zeo-ORF66-2xStrep

   Plasmid

   #130957

   Purpose

   Lentiviral vector for expression of KSHV ORF66 with a C-terminal 2xStrep tag

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG

   Plasmid

   #129758

   Purpose

   Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Glutamic acid 36 to Alanine and Leucine 3…

   Promoter

   TRE3Gs and Tet inducible promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. r-22. pT7-7-SNCA-9Asn

   Plasmid

   #107297

   Purpose

   Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 Gly

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-9Asn (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. t2. pT7-7-SNCA-5Lys

   Plasmid

   #107294

   Purpose

   Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-5Lys (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. r-6. pT7-7-SNCA-2Asn

   Plasmid

   #107296

   Purpose

   Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 Gly

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-2Asn (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. r. pT7-7-SNCA-5Asn

   Plasmid

   #107295

   Purpose

   Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-5Asn (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. s2. pT7-7-SNCA-5Asp

   Plasmid

   #107293

   Purpose

   Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-5Asp (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. arginine biosensor cpFLIPR

    Plasmid

    #205746

    Purpose

    Robust FRET biosensor enabling sensitive, accurate l-arginine measurement at physiological temp

    Depositor

    Colin Jackson

    Article

    Whitfield et al Protein Sci. 2015 Jun 9. doi: 10.1002/pr

    Insert

    cpFLIPR
    

    Use

    Unspecified

    Tags

    6 x Histidine purification tag (N-terminal), Biot…

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE2-TetO-Monbr-Sox-like

    Plasmid

    #231530

    Purpose

    Expresses Monsiga brevicolis full-length Sox-like in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Sox-like
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXJ-His-CD274

    Plasmid

    #232659

    Purpose

    transient expresses PD-L1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXJ-His-CD86

    Plasmid

    #232666

    Purpose

    transient expresses CD86 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXJ-His-CD80

    Plasmid

    #232665

    Purpose

    transient expresses CD80 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CD80 (CD80 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

    Plasmid

    #229797

    Purpose

    Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem

    Plasmid

    #229793

    Purpose

    Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem

    Plasmid

    #229792

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGG-PIP55

    Plasmid

    #196793

    Purpose

    Contains PDF1.2 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    PLANT DEFENSIN 1.2 (PDF1.2 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACE SOX (ORF37) D221N E244Q

    Plasmid

    #192053

    Purpose

    Expresses a 2X catalytic mutant of (ORF37) D221N E244Q

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (D221N E244Q)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    2X catalytic mutant D221N E244Q

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTE5418_pMBP-GlcR

    Plasmid

    #221192

    Purpose

    Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    glcR (pden4400 from Paracoccus denitrificans)
    

    Use

    Tags

    10x His tag with E. coli maltose binding protein …

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and T7 promoter-lacO

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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