We narrowed to 3,800 results for: golden gate

1. pAIP4_1JL1

   Plasmid

   #234206

   Purpose

   Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1JL1 (rnhA Escherichia coli)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAIP4_3TOW

   Plasmid

   #234201

   Purpose

   Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3TOW (MVB12B Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAIP4_1KQX

   Plasmid

   #234181

   Purpose

   Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1KQX (rbp2a Zebrafish)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAIP4_1AKO

   Plasmid

   #234162

   Purpose

   Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1AKO (xthA Escherichia coli K-12)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAIP4_4NFB

   Plasmid

   #234161

   Purpose

   Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4NFB (PILRA Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Coxsackievirus A6 (CVA6) strain JLS10/HeN/CHN/2019 3C protease

   Plasmid

   #228638

   Purpose

   Bacterial expression of the Coxsackievirus 63 3C protease

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Coxsackievirus 63 3C protease
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219690

   Purpose

   Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
   

   Expression

   Plant

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

   Plasmid

   #219688

   Purpose

   Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2BP/p35S (BFP, mCherry, NeonGreen)
   

   Expression

   Plant

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219686

   Purpose

   Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
   

   Expression

   Plant

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MUT_S7-H1

    Plasmid

    #218396

    Purpose

    3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. IBMc233

    Plasmid

    #161939

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    

    Expression

    Bacterial

    Promoter

    PPhlF

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. IBMc050

    Plasmid

    #161938

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
    

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. IBMc318

    Plasmid

    #161941

    Purpose

    Carries Golden Gate substitution insert for ER-LBD domain-insertion mapping

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-G-ER-LBD-S-SapI
    

    Expression

    Bacterial

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGImC_V5-CSNK1A1

    Plasmid

    #92014

    Purpose

    Expresses V5-CSNK1A1, includes IRES-mCherry

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAGM4723:Ble:Cas9

    Plasmid

    #112208

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

    Depositor

    Thomas Mock

    Inserts

    Cas9
    SheBle
    

    Use

    CRISPR; Diatom, phaeodactylum

    Tags

    Phaeodactylum fcp promoter and terminator and YFP

    Mutation

    Phaeodactylum Fcp promoter

    Promoter

    Phaeodactylum Fcp

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

    Plasmid

    #228636

    Purpose

    Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus A71 3C protease isoform B
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002

    Plasmid

    #228639

    Purpose

    Bacterial expression of the Coxsackievirus 33 isoform B 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Coxsackievirus 33 isoform B 3C protease
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease

    Plasmid

    #228644

    Purpose

    Bacterial expression of the D68 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus D68 3C protease
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pMV_AA101

    Plasmid

    #245323

    Purpose

    Cas9 CRISPRa positive control guide; targets CD274

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD274 (CD274 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDB_510

    Plasmid

    #245337

    Purpose

    Cas9 CBE positive control guide; targets CD274

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD274 (CD274 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge