We narrowed to 5,134 results for: golden gate

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eOYW012

Plasmid

#238392

Purpose

TurboRFP constitutive Golden Gate construct

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

UNS1-P2m-U1d-TurboRFP-t0-UNSX

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_SP_SD4_AraC

Plasmid

#242143

Purpose

Contains an AraC gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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L0-T7 Empty target Tp901

Plasmid

#195885

Purpose

Level 0 Empty Tp901 target: attP_Tp901-BpiI-attB_Tp901 with golden gate linker in pBLUNT plasmid.

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Empty Tp901 Target

Use

Synthetic Biology

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

Plasmid

#178994

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

Plasmid

#178992

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTSB-FICAT_Payload-Esp3I

Plasmid

#179380

Purpose

Transposon vector prepared for Esp3I golden gate cloning of Transgene

Depositor

Marc Güell

Article

Pallares-Masmitja et al Nat Commun. 2021 Dec 3;12(1):7071. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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Sth_I-E array_GFP dropout

Plasmid

#178775

Purpose

Golden Gate GFP dropout vector to generate S. thermophilus type I-E single arrays

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

gfp dropout to generate S. thermophilus type I-E single arrays

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pZA52

Plasmid

#174765

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V with STOP codon

Use

Unspecified

Mutation

L17E/D481V + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA50

Plasmid

#174763

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V with STOP codon

Use

Unspecified

Mutation

D481V + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA51

Plasmid

#174764

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E with STOP codon

Use

Unspecified

Mutation

L17E + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA49

Plasmid

#174762

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbZAR1 (NbZAR1syn) with STOP codon

Use

Unspecified

Mutation

WT + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2365 - Intron GG1 - 250bp no PATC

Plasmid

#159883

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2345 - Intron GG2 - 250bp no PATC

Plasmid

#159884

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2366 - intron GG3 - 250bp no PATC

Plasmid

#159885

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLY133

Plasmid

#130961

Purpose

Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLY131

Plasmid

#130956

Purpose

Engineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLY132

Plasmid

#130960

Purpose

Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB3

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLY130

Plasmid

#130952

Purpose

Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB1

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ136-tRNA2.0

Plasmid

#158398

Purpose

Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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