We narrowed to 6,564 results for: proc

1. pET28a SnoopTag-MBP

   Plasmid

   #72323

   Purpose

   Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-MBP
   

   Use

   Tags

   N-terminal His6 tag. MBP is fused at the C-termin…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pscALPSpuro-Nipah V

   Plasmid

   #174228

   Purpose

   Expresses Nipah virus V

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   Nipah V
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC-GoldyTALEN

   Plasmid

   #38143

   Purpose

   destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

   Depositor

   Daniel Carlson

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

   Insert

   GoldyTALEN
   

   Use

   Tags

   AcV5 and FokI homodimer

   Expression

   Mammalian

   Mutation

   Truncate at N and C terminous

   Promoter

   miniCaggs

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiGuide_mCherry_sgEPAS1#1

   Plasmid

   #183122

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fus-BcLOV-mCh-SOS

   Plasmid

   #208275

   Purpose

   Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

   Depositor

   Lukasz Bugaj

   Article

   Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   Fus-BcLOV-mCh-SOS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mNeonGreen-Smad3

   Plasmid

   #172098

   Purpose

   Expresses fluorescently tagged human Smad3 in mammalian cells.

   Depositor

   Lea Goentoro

   Article

   Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   Smad 3 (Smad3 Mouse)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   C49S mutation in mCerulean

   Promoter

   CMV

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Neo-Bam E2F6

   Plasmid

   #37965

   Depositor

   Jacqueline Lees

   Article

   Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

   Insert

   E2F6 (E2F6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. RCIscript-GoldyTALEN

   Plasmid

   #38142

   Purpose

   destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

   Depositor

   Daniel Carlson, Stephen Ekker

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

   Insert

   GoldyTALEN
   

   Use

   Mrna transcription vector

   Tags

   AcV5 and FokI homodimer

   Expression

   Mutation

   Truncate at N and C terminous

   Promoter

   T3

   Available since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHACK(Gal4)-DONR(T2A-Cas9)

   Plasmid

   #194768

   Purpose

   To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   T2A-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-INPP4A

    Plasmid

    #171944

    Purpose

    Expresses INPP4A in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    INPP4A (INPP4A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBI-p53 wt/EGFP

    Plasmid

    #16543

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN_Tmcs

    Plasmid

    #25751

    Purpose

    Entry vector with TRE promoter followed by multiple cloning site.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. [UBC][firefly luciferase][target cloning][renilla luciferase]

    Plasmid

    #97190

    Purpose

    Reporter for translation activation via Pum.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    Firefly Luciferase
    Renilla Luciferase
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    UBC (ubiquitin C)

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

    Plasmid

    #64112

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. GAL 103Q+ProGFPp416

    Plasmid

    #15583

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt103Q + Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. Luc-SIRT1 3'UTR

    Plasmid

    #20379

    Depositor

    Charles Lowenstein

    Article

    Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    3'UTR of SIRT1

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pMSCV-FlagBcl10

    Plasmid

    #18718

    Depositor

    Jon Ashwell

    Article

    Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

    Insert

    B cell lymphoma 10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag

    Plasmid

    #209658

    Purpose

    Non-responsive control

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    cpSFGFP.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVE-eGFP

    Plasmid

    #52581

    Purpose

    Lentiviral transfer vector driving expression of eGFP in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDS24

    Plasmid

    #58952

    Purpose

    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

    Depositor

    Thomas Fox

    Article

    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93

    Insert

    Mitochondrially coded Arg8 protein
    

    Use

    Tags

    Expression

    Mutation

    Coding sequence synthetic in S. cerevisiae mitoch…

    Promoter

    n/a

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only