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Showing: 1441 - 1460 of 1608 results

  2. CmCitrine Lck w/ NosUTRs

    Plasmid
    #66785
    Purpose
    expression of fluorescent membranes in sea urchin primordial germ cells
    Depositor
    Amro Hamdoun
    Article
    Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10
    Insert
    Lck (LCK Human)
    Use
    Sea urchin, zebrafish, xenopus
    Tags
    3' UTR from sea urchin Nanos, 5' UTR fr…
    Expression
    Mutation
    Tandem repeat of LCK the N-terminal membrane targ…
    Promoter
    T7
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pET-30a(+)-Syk-tSH2-FX

    Plasmid
    #111272
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    interdomain A (Phe 119 to His 162) substituted by…
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111272-plasmid-map-sequence-id-215700

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  10. p426_Cas9_gRNA-R-ARS805a

    Plasmid
    #87408
    Purpose
    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87408-plasmid-map-sequence-id-193450

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  14. pMal_Abl-PRM 3R

    Plasmid
    #112086
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    Abl-PRM-3R (ABL1 Human)
    Use
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112086-plasmid-map-sequence-id-236089

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  19. pMAL-Abl-PRM 4R

    Plasmid
    #112087
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    Abl-PRM-4R (ABL1 Human)
    Use
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112087-plasmid-map-sequence-id-236084

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  20. pMal-Abl-PRM 5R

    Plasmid
    #112088
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    PRM-5R (ABL1 Human)
    Use
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112088-plasmid-map-sequence-id-236080

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Showing: 1441 - 1460 of 1608 results