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Plasmid#89257PurposeExpress STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only
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pICH42222
Plasmid#50328DepositorInsertCDS, phosphinothricin acetyl transferase, (S. hygroscopicus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutation; See De…PromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS313-PRP8
Plasmid#89966Purposecomplements prp8 genomic deletionDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationnonePromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUbiSXR
Plasmid#64403PurposeUbiquitin promoter driving synthetic xylanase (sXynA) gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.DepositorInsertxylanase
UseTagsExpressionPlantMutationcodon optimized for plant expressionPromoterPlant ubiquitin (Ubi-1) promoter (with untranslat…Available sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS314-PRP16
Plasmid#89971PurposePrp16 in a trp backbone complements prp16 genomic deletionDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationnonePromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB605-GBSSI
Plasmid#213867PurposeBinary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertGRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB560-GBSSI
Plasmid#213866PurposeBinary vector for the expression of GBBSI fused to the tagRFP in plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertGRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI
Plasmid#192113PurposeExpresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-BA.4/5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
XE5 Xwnt-5A Hind RNA
Plasmid#16872DepositorInsertXwnt-5A Hind RNA (wnt5a.S Frog)
UseXenopus expressionTagsExpressionMutationStarts with AA 14 (see comments)PromoterAvailable sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH-ePPE
Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ePPE
Plasmid#183095PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-PheRS
Plasmid#124116PurposeBacterial expression plasmid for PURE system componentsDepositorUseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlyRS-His
Plasmid#124110PurposeBacterial expression plasmid for PURE system componentsDepositorUseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
P307-FBP_6
Plasmid#139697PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
puc19-pCas13a
Plasmid#118963PurposeIntermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.DepositorInsertCas13a
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Pt-mChe
Plasmid#183164PurposeConstruct for transient expression of plastids-targeted mCherry in plants.DepositorInsertPt-mCherry
UseTags57 aa of the Pisum sativum rubisco small subunit …ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUbi-MAD7
Plasmid#170133PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRTagsExpressionPlantMutationPromotermaize Ubiquitin-1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
P362-FBP_12
Plasmid#139703PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)
Plasmid#136627PurposePlasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-LAMA-R59
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES EGFP
Plasmid#136620PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-G97-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only