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pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233033

Purpose

To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Insert

DjCas13d and mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233034

Purpose

To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Insert

hfCas13d and mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233032

Purpose

To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Insert

CasRx and mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2

Plasmid

#231208

Purpose

Single transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)

Use

Lentiviral

Tags

Expression

Mutation

L80F, R81D, L85V, I86V, I92F

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2

Plasmid

#231207

Purpose

Single transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Use

Lentiviral

Tags

Expression

Mutation

L147I, R148H, I203V, L290V

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

Plasmid

#197989

Purpose

For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNT0217290

Use

Lentiviral

Tags

lac

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

Plasmid

#226590

Purpose

Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.

Depositor

D. James Surmeier

Insert

PSAML-141F,Y115F-GlyR

Use

AAV

Tags

P2A-Fusion Red

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)

Plasmid

#199582

Purpose

Expresses Chronos-eGFP in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Chronos eGFP

Use

AAV, CRISPR, and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a intron A

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

Plasmid

#195018

Purpose

An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

Depositor

Christopher Richie

Article

Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

Inserts

CCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG

Use

AAV

Tags

KASH domain of Nesprin2

Expression

Mutation

Promoter

EF1a, hU6, and mU6

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

Plasmid

#216477

Purpose

lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

dsRed
tagBFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

Plasmid

#204214

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of DNA binding domain (delDBD)

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

Plasmid

#204191

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of R162

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

Plasmid

#204213

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Point mutation on I207M

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204211

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Point mutation on Q175* (*termination)

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204210

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Point mutation on R178* (*termination)

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

Plasmid

#204212

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NK2 homeobox 1 (NKX2-1 Human)

Use

Lentiviral

Tags

Point mutation on I207F

Expression

Mammalian

Mutation

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Degradation Domain

Plasmid

#184692

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Degradation Domain

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Disordered Region

Plasmid

#184693

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Disordered Region

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Disordered Region

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Rad23b UbL

Plasmid

#184694

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbL

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Rad23b UbL

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen delta Degradation Domain

Plasmid

#184701

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Degradation Domain

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge