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pGEX6P1-A20 (OTU, aa 1-366)

Plasmid

#61584

Purpose

Expresses human A20 (OTU domain) in E. coli.

Depositor

David Komander

Article

Komander et al Biochem J. 2008 Jan 1;409(1):77-85.

Insert

A20 (TNFAIP3 Human)

Tags

GST-3C

Expression

Bacterial

Mutation

Deleted aa 367-790.

Promoter

tac

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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FLM-Q4225P

Plasmid

#41582

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

FLM Q4225P

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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CBX2

Plasmid

#25158

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CBX2_04 (CBX2 Human)

Tags

6x His and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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FLM-R4154C

Plasmid

#41580

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

FLM R4136G

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-S4190F

Plasmid

#41581

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

FLM S4190F

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
POC1520

Plasmid

#221581

Purpose

pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1519

Plasmid

#221580

Purpose

pMOBK360_VM: Vector Middle (VM) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-STOP

Plasmid

#215840

Purpose

Expression of FLAG/HA tag

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0522

Plasmid

#196158

Purpose

Phytobricks Level 0 part: MinSyn_305

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_305

Use

Synthetic Biology

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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bpNLS-DddIA

Plasmid

#187847

Purpose

expresses the bpNLS-linked DddIA in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

bpNLS-DddIA-bpNLS-p2A-EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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FBXO7_Halo_C_allele

Plasmid

#178158

Purpose

Donor vector for endogenous tagging of human FBXO7 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (FBXO7 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFGL1269R

Plasmid

#118994

Purpose

Marker fusion of Bar:mCherry (without start codon)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

Bar:mCherry

Use

Fungal expression

Promoter

promoter less

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1259

Plasmid

#118996

Purpose

GFP (without start and stop codons) in pFGL1010

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

eGFP

Use

Fungal expression

Promoter

promoter less

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJK593

Plasmid

#71587

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJK594

Plasmid

#71588

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with C-terminal His6 tag (YfdEH6)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 to gap

Plasmid

#49158

Purpose

Template for generating gene 60 mRNA, sequence 5' of coding gap only

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Mutation

Coding gap and downstream sequence have been del…

Promoter

T7

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM-T Dmel Or49a

Plasmid

#59661

Purpose

Probe for Drosophila melanogaster Or49a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or49a (Or49a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pNH052

Plasmid

#42158

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

YFP-RT-YFP

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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HSP104-b/Leu(G217S/T499I)

Plasmid

#1158

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Tags

GAL1:10 promoter

Expression

Yeast

Mutation

G217S/T499I

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pTP1112

Plasmid

#104158

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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