We narrowed to 1,873 results for: FRT

1. Fireworks GFP[PTC+] (AVA2600)

   Plasmid

   #85446

   Purpose

   Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FDIO-COX4-dAPEX2

   Plasmid

   #117178

   Purpose

   Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   COX4-dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAV1-mEGFP-mCherry

   Plasmid

   #27763

   Depositor

   Ari Helenius

   Article

   Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Use

   Tags

   monomeric EGFP-monomeric Cherry (tandem)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-mTIP49a

   Plasmid

   #15357

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   TATA binding protein interacting protein 49 kDa (Ruvbl1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-FDIO-LACTB-dAPEX2

   Plasmid

   #117181

   Purpose

   Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   LACTB-dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3'-3xFLAG minimal CMV pCDNA5

   Plasmid

   #212045

   Purpose

   LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. BCL9-BirA*

   Plasmid

   #109147

   Purpose

   To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

   Depositor

   Mariann Bienz

   Article

   van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

   Insert

   BCL9 (BCL9 Human)
   

   Use

   Tags

   BirA(R118G)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-Amida

   Plasmid

   #15362

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   TCF3 (E2A) fusion partner (TFPT Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

9. Sox17(2A-phiC31o)

   Plasmid

   #140762

   Purpose

   Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   phiC31o
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Endogenous Sox17

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sox1(2A-Cre)

    Plasmid

    #140760

    Purpose

    Targeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    Cre
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Endogenous Sox1

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgFFL

    Plasmid

    #59877

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Use

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FDIO-SYP-HRP

    Plasmid

    #117187

    Purpose

    Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    SYP-HRP
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5 HA-Amida

    Plasmid

    #19794

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    Amida (TFPT Human)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 24, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5-MIS12-LAP-BUB1

    Plasmid

    #115904

    Purpose

    Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

    Insert

    MIS12-BUB1 (MIS12 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    BUB1 fused to MIS12

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-PPP2R5E siRNA-resistant

    Plasmid

    #120861

    Purpose

    expresses dox-inducible PPP2R5E resistant to siRNA pool 2 used in manuscript

    Depositor

    Emily Foley

    Article

    Lee et al J Cell Sci. 2017 May 15;130(10):1760-177

    Insert

    PPP2R5E (PPP2R5E Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation at aa 282-284 in PPP2RE to genera…

    Promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEXP(FLAG/HA-NSP1-WT)

    Plasmid

    #188781

    Purpose

    Expresses FLAG/HA-NSP1 in mammalian cells (SARS-CoV-2 NSP1)

    Depositor

    Markus Hafner

    Article

    Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do

    Insert

    FLAG/HA-NSP1 (SARS-CoV-2)
    

    Use

    Frt site to insert into hek293 t-rex genome

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Dox inducible

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3'-BirA*-FLAG minimal CMV pCDNA5

    Plasmid

    #212047

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 5'-BirA*-FLAG minimal CMV pCDNA5

    Plasmid

    #212046

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 5'-3xFLAG GFP minimal CMV pCDNA5

    Plasmid

    #212086

    Purpose

    LR vector for integration of GFP into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BirA-Myc_N_FOXL1

    Plasmid

    #167703

    Purpose

    Expresses FOXL1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    FOXL1 (FOXL1 Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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