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We narrowed to 6,501 results for: nls

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Showing: 1461 - 1480 of 6501 results

  2. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid
    #87827
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Publication
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87827-plasmid-map-sequence-id-175008

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  5. MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176627
    Purpose
    TF B self-activation cassette in MultiFate-2.2 and MultiFate-3
    Depositor
    Michael Elowitz
    Publication
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176627-plasmid-map-sequence-id-347070

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  7. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Publication
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

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  8. pET-dHeFSpCas9-VP64-6xHis

    Plasmid
    #92119
    Purpose
    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, NLS, and VP64
    Expression
    Bacterial
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    T7
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92119-plasmid-map-sequence-id-194893

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  9. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid
    #131312
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    131312-plasmid-map-sequence-id-254654

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  10. IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

    Plasmid
    #121824
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sa-dCas9/LSD1 (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121824-plasmid-map-sequence-id-237376

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  11. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid
    #107305
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2x NLS and FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107305-plasmid-map-sequence-id-204646

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  12. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

    Plasmid
    #89587
    Purpose
    For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.
    Depositor
    Takuji Iwasato
    Publication
    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nuclear localization signal (nls) turboRFP
    Expression
    Mammalian
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    89587-plasmid-map-sequence-id-180320

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  13. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176624
    Purpose
    TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3
    Depositor
    Michael Elowitz
    Publication
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176624-plasmid-map-sequence-id-347134

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  14. MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA

    Plasmid
    #176629
    Purpose
    TF C self-activation cassette in MultiFate-3
    Depositor
    Michael Elowitz
    Publication
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
    Use
    Synthetic Biology
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176629-plasmid-map-sequence-id-347269

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  16. pcDNA3-nuc-Nb80

    Plasmid
    #205519
    Purpose
    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Publication
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb80
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205519-plasmid-map-sequence-id-409422

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  17. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid
    #185706
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS
    Depositor
    Raul Perez-Jimenez
    Publication
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Tags
    6xHis-NLS and NLS
    Expression
    Mammalian
    Mutation
    optimized for human codon usage
    Promoter
    CMV and T7
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185706-plasmid-map-sequence-id-363635

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  18. BM00654

    Plasmid
    #155339
    Purpose
    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.
    Depositor
    Matias Zurbriggen
    Publication
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    Use
    Luciferase and Synthetic Biology
    Expression
    Plant
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155339-plasmid-map-sequence-id-360426

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  19. pKL078

    Plasmid
    #234944
    Purpose
    Constitutive expression of membrane receptor Lck linked to mNeonGreen fluorescent protein and a nuclear localized mTagBFP2 fluorescent protein
    Depositor
    Martin Kampmann
    Publication
    Teter et al Mol Psychiatry. 2025 Sep;30(9):4176-4193
    Insert
    Lck-mNeonGreen and NLS-mTagBFP2
    Use
    Lentiviral
    Tags
    Fusion
    Available since
    Sept. 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    234944-plasmid-map-sequence-id-495007

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Showing: 1461 - 1480 of 6501 results