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We narrowed to 6,178 results for: nls

Showing: 1461 - 1480 of 6178 results

  1. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  5. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51086
    Purpose
    Forebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Promoter
    CaMKIIa 1.3 kb
    Available Since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    51086-plasmid-map-sequence-id-469612

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  9. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

    Plasmid
    #164614
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    stdMCP-stdHalo-RH1
    Use
    Lentiviral
    Tags
    HA tag and NLS
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164614-plasmid-map-sequence-id-325003

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  12. phage - ubc - nls - 2xmcp - mCherry - BirA*

    Plasmid
    #131136
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-mCherry-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131136-plasmid-map-sequence-id-255502

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  13. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

    Plasmid
    #87803
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87803-plasmid-map-sequence-id-175010

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  17. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid
    #89993
    Purpose
    Donor template for generation of SOX9-ntdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    Use
    CRISPR
    Promoter
    none
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    89993-plasmid-map-sequence-id-180698

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Showing: 1461 - 1480 of 6178 results