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pJAF205

Plasmid

#60407

Purpose

Flag-tagged full length GMAP210/Trip11

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2008 Dec;4(12):e1000315. doi

Insert

GMAP210 (Trip11 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pLPCX mito roGFP2-Orp1

Plasmid

#64992

Purpose

Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-TAZ-N-Flag

Plasmid

#19025

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pLD1 translation factors

Plasmid

#117760

Purpose

BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 1-13

Use

Escherichia coli

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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eMagB-iRFP-Mito

Plasmid

#162250

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-iRFP-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-NeonGreen

Plasmid

#139364

Purpose

FRET Standard

Depositor

Richard Day

Article

Reissaus et al Biophys J. 2020 Apr 21;118(8):1820-1829.

Insert

Scarlet 10AA Neon green

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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Cys-30nmSAH-SPYcatcher

Plasmid

#214839

Purpose

This plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Cys-30nmSAH-SPYcatcher

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry- eMagBF - 5ptaseOCRL

Plasmid

#162254

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

mCherry- eMagBF - 5ptaseOCRL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pQE-60 roGFP2-Orp1-His

Plasmid

#64976

Purpose

Bacterial expression of His-tagged roGFP2-Orp1

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Tags

His and roGFP2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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Lys-eMagB-iRFP

Plasmid

#162249

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

p18-eMagB-iRFP

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-H2GH

Plasmid

#182202

Purpose

Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

histone2B-HaloTag-GFP

Use

AAV

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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Cyto-ABKAR

Plasmid

#61510

Purpose

Expression of AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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ER-EGFP-eMagA

Plasmid

#162245

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-EGFP-eMagA

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Mito-ABKAR

Plasmid

#61509

Purpose

Expression of AMPK biosensor targeted to the mitochondria

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Mitochondrial targeting signal (DAKAP1-30)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
AAV-HGM (optimized)

Plasmid

#220988

Purpose

Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

HaloTag-GFP-mito

Use

AAV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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eMagA-mCherry-Mito

Plasmid

#162251

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-mCherry-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET30a-GlmU-NahK-ET64

Plasmid

#234663

Purpose

Expression an ELP-tagged fusion enzyme of GlmU and NahK

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

N-acetylglucosamine- 1-phosphate uridyltransferase
N-acetylhexosamine 1-kinase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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