161,152 results

1. pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156982

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PTGFRN (PTGFRN Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR/zeo-MACROD2

   Plasmid

   #172573

   Purpose

   For gateway cloning of N-terminal tagged MACROD2 constructs.

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-anti-mCherry(LaM6) PAGER(Gq)

   Plasmid

   #230016

   Purpose

   Lentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaM6-TEVcs-ALFA-hM1D
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   W442A on hM1D

   Promoter

   CMV

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-anti-mCherry(LaM6) PAGER(Gs)

   Plasmid

   #230017

   Purpose

   Lentiviral vector expressing anti-mCherry PAGER(Gs) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaM6-TEVcs-ALFA-hM1Dxs
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-tagBFP

   Plasmid

   #217007

   Purpose

   lentiviral plasmid for gRNA cloning with selectable tagBFP expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLifeAct-mTurquoise2

   Plasmid

   #36201

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   LifeAct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-ChrimsonR-tdT

   Plasmid

   #59171

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

   Plasmid

   #79650

   Purpose

   Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein

   Depositor

   Jeffrey Friedman

   Article

   Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

   Inserts

   transient receptor potential vanilloid 1 (Trpv1 Rat)
   camelid anti-GFP nanobody
   Enhanced green fluorescent protein
   mouse ferritin light chain (Ftl1 Mouse)
   mouse ferritin heavy chain (Fth1 Mouse)
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   isoleucine 679 changed to lysine

   Promoter

   CMV

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-FLAG-HA-hYTHDC1

   Plasmid

   #85167

   Purpose

   Expression of FLAG-HA-tagged human YTHDC1

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Tags

   Flag and Ha

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti PGK FKBP12(F36V)-OGT (Neo)

    Plasmid

    #154294

    Purpose

    Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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