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pExchange_tdk_BT1311p-tetR-2

Plasmid

#90326

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-miR-17-5p-Control

Plasmid

#21167

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

miR 17-5p Sensor (MIR17 Human)

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4C01

Plasmid

#71009

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Cand1 (CG5366 Fly)

Use

Gateway Cloning

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
RSV CREB

Plasmid

#22394

Depositor

Marc Montminy

Article

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
Gal-Rad51

Plasmid

#33230

Depositor

Hannah Klein

Article

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Expression

Yeast

Promoter

Gal1

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pIA1364

Plasmid

#172401

Purpose

Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp9 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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I-2/pCS2MT

Plasmid

#16317

Depositor

Peter Klein

Article

Zhang et al J Biol Chem. 2003 Aug 29. 278(35):33067-

Insert

Inhibitor-2 (Ppp1r2 Mouse)

Use

Vertebrate expression

Tags

5x myc

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pT2KXIGdeltaIn-MCS-birA-tagRFP

Plasmid

#58378

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA tagRFP

Tags

tagRFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-birA-EGFP

Plasmid

#58377

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA EGFP

Tags

EGFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSuper-Sema4A 42-3n

Plasmid

#115173

Purpose

shRNA against rodent Sema4A

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

shRNA targeting Sema4A

Use

RNAi

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+Flag-XTCF3-HA

Plasmid

#32998

Depositor

Sergei Sokol

Article

Hikasa et al Dev Cell. 2010 Oct 19;19(4):521-32.

Insert

XTCF3 (tcf3 Frog)

Tags

Flag and HA

Expression

Mammalian

Promoter

sCMV IE94

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pIA1405

Plasmid

#172404

Purpose

Expression of SARS-COV-2 Nsp12 D218A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

D218A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1420

Plasmid

#172406

Purpose

Expression of SARS-COV-2 Nsp12 R116A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

R116A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1421

Plasmid

#172407

Purpose

Expression of SARS-COV-2 Nsp12 K50A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMJB1

Plasmid

#160429

Purpose

Yeast vector for expressing mCherry tagged genes under the GAL1 promoter

Depositor

Gary Stormo

Article

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Type

Empty backbone

Tags

mCherry

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pIA1402

Plasmid

#172403

Purpose

Expression of SARS-COV-2 Nsp12 Y129A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

Y129A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCTX-myc-HIPK2 KD

Plasmid

#32997

Depositor

Sergei Sokol

Article

Hikasa et al Dev Cell. 2010 Oct 19;19(4):521-32.

Insert

HIPK2 (hipk2 Frog)

Tags

6XMyc

Mutation

K217>R (in the catalytic lysine) and STY348-35…

Promoter

SCMV

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B09

Plasmid

#70833

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

rhi (rhi Fly)

Use

Gateway Cloning

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pIA1408

Plasmid

#172405

Purpose

Expression of SARS-COV-2 Nsp12 D760A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

D760A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2

Plasmid

#60388

Purpose

Expression of yeast beta tubulin (TUB2) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only