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Plasmid#106456PurposeNF-kB p65, kinase sites 276,305 mutatedDepositorInsertNF-kB p65, kinase sites 276,305 mutated (RELA Human)
UseTagsExpressionMammalianMutationkinase sites inactivatedPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ
Plasmid#51495PurposepLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmidDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant t-dna plasmidTagsSV40 NLSExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-eGFP-Esp3I
Plasmid#81226PurposeCloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sitesDepositorInsertEsp31-Neo-Esp31 deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch10
Plasmid#81224PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-B/c
Plasmid#227962PurposeEntry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.DepositorInsertB/c
UseTagsExpressionMutationPromoterNoneAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-LacI-mHP1a
Plasmid#232637Purposelac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alphaDepositorInsertHaloTag-LacI-mHP1a (Cbx5 Mouse, Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
PAC_E83_LOV_eGFP
Plasmid#231574PurposeThis plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue lightDepositorInsertPAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Galt1-HT7-mGold
Plasmid#230027PurposeExpressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.DepositorInsertGalt1-HaloTag7-mGold (B4GALT1 Synthetic, Human)
UseTagsHaloTag,mGoldExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
RcSxph
Plasmid#194018PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionMutationPromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-pDHS-ITDE-AP1x6-DUSP5
Plasmid#217419PurposeLuciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDeDepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterHuman DUSP5 promoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF2 CTF-FLAG
Plasmid#217695PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF2 (ADGRF2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-429PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE1 CTFdeltaTA-FLAG
Plasmid#217722PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE1 (EMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-590PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE4 CTF-FLAG
Plasmid#217692PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE4 (ADGRE4P Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-173PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF2 CTFdeltaTA-FLAG
Plasmid#217728PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF2 (ADGRF2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-435PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA1-FLAG
Plasmid#217678PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA1 (ADGRA1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
BcrMT1B Blue Crab Metallothionein 1B
Plasmid#200290PurposeBacterial expression plasmids for production of recombinant Blue Crab Callinectes sapidus Metallothionein 1BDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato. PGK-puro
Plasmid#136264PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMiR-HPV16-E7/E1-miR-375-m1-5
Plasmid#53704PurposeLuciferase reporter assay for HPV16 E7/E1 with point mutation on miR-375 binding siteDepositorInsertHPV16-E7/E1
UseLuciferaseTagsExpressionMutationMutation in miR-375 binding sites m1-5 (refer to …PromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys
Plasmid#15602DepositorInsertSup35 NM (SUP35 Budding Yeast, Candida albicans)
UseTags7xHis and CysteineExpressionBacterialMutation4 G/A. Four glycines mutated to alanines. The pos…PromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
EGFP-alphasynuclein-A53T
Plasmid#40823DepositorInsertSNCA (SNCA Human)
UseTagsEGFPExpressionMammalianMutationA53T (aggregates more rapidly than the wild-type …PromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBBR1MCS-2
Plasmid#85168PurposeMobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsExpressionBacterialMutationPromoterConstitutive (derepressed P-lac)Available sinceNov. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV_pMBP-EGFP-caax
Plasmid#190155PurposeExpresses membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertEGFP-caax
UseAAVTagsExpressionMammalianMutationPromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
4XCLEAR-luciferase reporter
Plasmid#66800PurposeReporter for activity of the CLEAR networkDepositorInsert4XCLEAR
UseLuciferaseTagsExpressionMutationPromoterFour CLEAR elements (GTCACGTGAC) in tandem derive…Available sinceNov. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EGFP-Bulged-miR-19
Plasmid#91976PurposeEGFP reporter cell line with eight bulged miR-19 sitesDepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFPc2 mAbp1
Plasmid#26719DepositorInsertActin-binding Protein-1 (Dbnl Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-Myc
Plasmid#177717PurposeER protein alpha-1-antitrypsin (WT), myc taggedDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsMycExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT-mGold
Plasmid#183987PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag, mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro
Plasmid#136262PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreen-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP
Plasmid#60975PurposeRapid selection of recombinant marker-free fluorescent rodent malaria parasitesDepositorInsertsGFP
mCherry
hDHFR-yFCU
UsePlasmodium gene targetingTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only