We narrowed to 21,668 results for: Tes

1. BLA_CAT_K136_GPG_LOV2

   Plasmid

   #231575

   Purpose

   This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   CAT_ carrying an AsLOV2 insertion behind K136
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG

   Plasmid

   #217725

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE4
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-179

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. H2B-HT-emiRFP703

   Plasmid

   #183985

   Purpose

   Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

   Depositor

   Pablo Rivera-Fuentes

   Article

   Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   HaloTag, emiRFP703

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. H2B-HT-mGold

   Plasmid

   #183988

   Purpose

   Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

   Depositor

   Pablo Rivera-Fuentes

   Article

   Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   HaloTag, mGold

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-Isce-Tol2-Tn5-neo

   Plasmid

   #176619

   Purpose

   for generating PCR templates with Tol2 repeats and Isce sites for BAC recombination

   Depositor

   Nathan Lawson

   Article

   Shih et al Development. 2021 Nov 9. pii: 273393. do

   Insert

   I-SceI sites, Tol2 repeats and Tn5-neo cassette
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_FokI-1x5

   Plasmid

   #63605

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-myc-Rem2 RNAiR2 short

   Plasmid

   #51596

   Purpose

   expresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2 Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   silent mutations at shRNA 1 and 2 recognition sit…

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-myc-Rem2 R236A/L263A

   Plasmid

   #51589

   Purpose

   expresses RNAiR myc-tagged Rem2 non-Ca channel interacting

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R236A and L263A; silent mutations at shRNA 1 and …

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-myc-Rem2 S69A/S334A

   Plasmid

   #51588

   Purpose

   expresses RNAiR myc-tagged Rem2 non-14-3-3 interacting

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S69A and S334A; silent mutations at shRNA 1 and 2…

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sup35 Sc/Ca NM Chimera S17R His7 Cys

    Plasmid

    #15601

    Depositor

    Susan Lindquist

    Article

    Tessier et al Nature. 2007 May 31. 447(7144):556-61.

    Insert

    Sup35 NM (SUP35 Budding Yeast, Candida albicans)
    

    Use

    Tags

    7xHis and Cysteine

    Expression

    Bacterial

    Mutation

    S17R. Serine 17 mutated to arginine.

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. WT Ecm2

    Plasmid

    #169922

    Purpose

    Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. PiggyBac dCas9-KRAB

    Plasmid

    #191314

    Purpose

    piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    KRAB-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUGW-d2GFP-200

    Plasmid

    #79602

    Purpose

    FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    d2GFP + miR-200 family targets
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-IRES-Thy1.1 DEST

    Plasmid

    #17442

    Purpose

    Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. GfaABC1D-jGCaMP8m

    Plasmid

    #213010

    Purpose

    To study calcium signaling in Astrocytes

    Depositor

    Baljit Khakh

    Article

    Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

    Insert

    GfaABC1D-jGCaMP8m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMTS-HA-GFP11

    Plasmid

    #177725

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-HA-GFP11
    

    Use

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMTS-hFluc

    Plasmid

    #177716

    Purpose

    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    Mitochondrial Targeting Sequence MTS SS

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV 4xSTAR-mNeonGreen-NLS-blast

    Plasmid

    #136258

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA 3.1 Flag-rat-PTP1B

    Plasmid

    #35988

    Depositor

    Anna Huttenlocher

    Article

    Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

    Insert

    protein tyrosine phosphatase 1B (Ptpn1 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAD-GamS

    Plasmid

    #99246

    Purpose

    Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

    Depositor

    Jeff Hasty

    Article

    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

    Insert

    gamS (with C-terminal 6xHis tag)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Oct. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. MSCV-EGFP

    Plasmid

    #91975

    Purpose

    EGFP reporter cell line control (no miR-19 sites)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMCSG53-DsbC

    Plasmid

    #186623

    Purpose

    E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

    Depositor

    Alexei Savchenko

    Article

    Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

    Type

    Empty backbone

    Use

    Tags

    DsbC-6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Galt1-Grx1-roGFP1-iE

    Plasmid

    #230029

    Purpose

    Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Galt1-Grx1-roGFP1-iE (B4GALT1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV 4xSTAR-sTomato-NLS-blast

    Plasmid

    #136259

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-Ran-T24N-mRFP1-polyA

    Plasmid

    #104561

    Purpose

    Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    T24N

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV 4xSTAR-mScarletI-NLS-blast

    Plasmid

    #136260

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mScarletI-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-Ran-Q69L-mRFP1-polyA

    Plasmid

    #104560

    Purpose

    Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Q69L

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LTR5HS S. aureus scaffold CARGO array

    Plasmid

    #191317

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Galt1-sCGrx1p-HA

    Plasmid

    #230028

    Purpose

    Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Galt1-sCGrx1p-HA (B4GALT1 Human, Synthetic)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge