We narrowed to 7,241 results for: 11

1. pMSCV-MAVS-M142A(KaganE19)

   Plasmid

   #52010

   Purpose

   Expresses the human MAVS CDS containing the point mutation M142A

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-M142A
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   changed Met 142 to Ala

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-MAVS-M1A,M142A(KaganF62)

   Plasmid

   #52002

   Purpose

   Expresses the human MAVS CDS containing two point mutations M1A and M142A

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS
   

   Expression

   Mammalian

   Mutation

   changed Met 1 to Ala and Met 142 to Ala

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-MAVS-M142A(KaganF59)

   Plasmid

   #52062

   Purpose

   Expresses the human MAVS CDS containing the point mutation M142A "weak translational context"

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-M142A
   

   Expression

   Mammalian

   Mutation

   changed Met 142 to Ala

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-Xl-VSP2 GFP

   Plasmid

   #51886

   Purpose

   expression of Xl-VSP2-GFP fusion in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP2
   

   Use

   Xenopus oocyte expression

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICSL00002

   Plasmid

   #50347

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   Linker for joining modules with 3' TTCG overhangs to modules with 5' GTGG overhangs
   

   Use

   Plant expression

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL00001

   Plasmid

   #50346

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   Linker for joining modules with 3' GTGG overhangs to modules with 5' TTCG overhangs
   

   Use

   Plant expression

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQC_HA-p115 TEV-Myc #77#407

   Plasmid

   #42127

   Depositor

   Ayano Satoh

   Article

   Koreishi et al Antibody Technology Journal. 2011;1:5-11

   Insert

   p115
   

   Use

   Retroviral

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   inserted ENLYFQ b/w 886 and 887

   Promoter

   CMV

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

8. 27 pCR plcg 1 CDS

   Plasmid

   #13058

   Depositor

   Nathan Lawson

   Article

   Lawson et al Genes Dev. 2003 Jun 1. 17(11):1346-51.

   Insert

   plcg1 (plcg1 Zebrafish)
   

   Available Since

   June 18, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pMT Draper-delta ITAM-GFP

   Plasmid

   #126655

   Purpose

   Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper with ITAM Tyr residues mutated to Ala
   

   Expression

   Insect

   Mutation

   Cytoplasmic ITAM Tyr residues mutated to Phe

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His10-Draper delta ITAM

    Plasmid

    #126671

    Purpose

    Expression of His10-Draper delta ITAM mutant for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper cytoplasmic ITAM residues mutated to Phe
    

    Expression

    Bacterial

    Mutation

    Cytoplasmic ITAM residues mutated to Phe

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-PPO-mScarlet

    Plasmid

    #139503

    Purpose

    Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    parapinopsin
    

    Tags

    mScarlet following Gly-Gly-Ser linker

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNGVL4a-hCRTmE6mE7mL2

    Plasmid

    #104153

    Purpose

    Expresses human calreticulin fused to MmuPV1 E6, MmuPV1 E7 and MmuPV1 L2 residues 11-210 in mammalian cells

    Depositor

    Richard Roden

    Article

    Jiang et al J Virol. 2017 Jul 12;91(15). pii: e00699

    Insert

    hCRTmE6mE7mL2 11-210 (CALR, E6, E7, L2 Human, MmuPV1)
    

    Expression

    Mammalian

    Mutation

    residues 11 to 200 of the late protein L2

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA3-PPO-Venus

    Plasmid

    #139502

    Purpose

    Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    parapinopsin
    

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-mScarlet-gamma9

    Plasmid

    #140097

    Purpose

    Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    GNGT2
    

    Tags

    N-terminal mScarlet tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSV-Rev

    Plasmid

    #12253

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    Rev (rev )
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPRv2 sgSp1

    Plasmid

    #174907

    Purpose

    Second generation sgRNA against Sp1

    Depositor

    Jane Clifford

    Article

    Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

    Insert

    Specificity Protein 1 (SP1 Human)
    

    Expression

    Mammalian

    Mutation

    Missense mutation to mutate PAM sequence

    Promoter

    EFS-NS

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRIS-PITChv2-FBL

    Plasmid

    #63672

    Purpose

    PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    EGFP-2A-PuroR
    

    Use

    CRISPR

    Promoter

    Promoterless

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330S-2-PITCh

    Plasmid

    #63670

    Purpose

    Expresses Cas9 nuclease and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

    Plasmid

    #188703

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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