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pdTCas9_scr

Plasmid

#207568

Purpose

dThermoCas9-mediated silencing of genomic gene in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_dThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

ThermoCas9(D8A,H582A)

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pdGCas9_scr

Plasmid

#207569

Purpose

dGeoCas9-mediated silencing of genomic gene in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_dGeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

GeoCas9(D8A,H582A)

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAcrTCas9_scr

Plasmid

#207571

Purpose

AcrIIC1-mediated inhibition of ThermoCas9 cleavage activity in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Prha_AcrIIC1Nme_Plac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAcrGCas9_scr

Plasmid

#207572

Purpose

AcrIIC1-mediated inhibition of GeoCas9 cleavage activity in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Prha_AcrIIC1Nme_Plac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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H2B-mScarlet3-S2

Plasmid

#246872

Purpose

Expression of mScarlet3-S2. This contruct is used for imaging, photobleaching and photochromic behavior measurements.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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mScarlet3-S2-Lamin A/C

Plasmid

#246875

Purpose

Expression of mScarlet3-S2 fused with Lamin A/C. This construct is used for imaging.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CP2

Plasmid

#223334

Purpose

Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

Toc64-EGFP-TurboID-3HA

Tags

EGFP-TurboID-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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CP4

Plasmid

#223336

Purpose

Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

Toc64-EGFP-miniTurboID-3HA

Tags

EGFP-miniTurboID-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1-Cdc45-6His

Plasmid

#211379

Purpose

bacterial expression of human Cdc45 C-terminally fused to 6xHis-Tag

Depositor

Helmut Pospiech

Article

Szambowska et al Nucleic Acids Res. 2014 Feb;42(4):2308-1

Insert

Cdc45 (CDC45 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pT2/shFYN1/GFP4_Seq_B

Plasmid

#206135

Purpose

Knockdown of FYN

Depositor

Maria Castro

Article

Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

Insert

FYN shRNA (Fyn Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shFYN1/GFP4_Seq_A

Plasmid

#206134

Purpose

Knockdown of FYN

Depositor

Maria Castro

Article

Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

Insert

FYN shRNA (Fyn Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-IGFBP2-Clover

Plasmid

#191007

Purpose

Gateway entry vector with IGFBP2 tagged with Clover

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Gateway entry vector

Tags

Clover

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pOBD2

Plasmid

#196826

Purpose

Yeast two-hybrid ; Gal4 DNA binding domain

Depositor

Stan Fields

Article

Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

Type

Empty backbone

Tags

Gal4 DNA binding domain

Expression

Yeast

Promoter

ADH

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199453

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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Chinchilla–insM

Plasmid

#191226

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed methionine at 122 in the protein sequence

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Sandgrouse–insD

Plasmid

#191228

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed aspartic acid at 121 in the protein seque…

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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GLE_sgRNA

Plasmid

#186658

Purpose

GLE sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Gle sgRNA Plasmid (CG14749 Fly)

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Shu_sgRNA

Plasmid

#186656

Purpose

shu sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Shu sgRNA Plasmid (shu Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_nonPuro_OSS

Plasmid

#186652

Purpose

Cas9 plasmid to express the Cas9 in OSS cells

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Cas9 Expression Plasmid

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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VIR_sgRNA

Plasmid

#186661

Purpose

VIR sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

VIR sgRNA Plasmid (vir Fly)

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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