We narrowed to 1,518 results for: pet-28a

1. Human HUWE1 (3993-4374)

   Plasmid

   #110754

   Purpose

   Bacterial Expression of HUWE1 HECT domain

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHelA12

   Plasmid

   #176493

   Purpose

   Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

   Depositor

   Jian-Dong Huang, Xiaodong Cheng

   Article

   Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

   Insert

   SARS-CoV NSP13 (ORF1ab SARS-CoV)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   V570I

   Promoter

   T7

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK597

   Plasmid

   #71121

   Purpose

   Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

   Depositor

   T. J. Kappock

   Article

   Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

   Insert

   formyl-CoA:oxalate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p47 PX(1-121)/p40phox(136-339)

   Plasmid

   #19018

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox/p47phox (NCF4, NCF1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   insert containing amino acids 1-121 of p47phox fu…

   Promoter

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

5. hSTX4(1-271,272C)

   Plasmid

   #206031

   Purpose

   Bacterial expression of human Syntaxin 4 without transmembrane helix and a C-terminal cysteine

   Depositor

   Geert van den Bogaart

   Article

   Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

   Insert

   Syntaxin-4 (STX4 Human)
   

   Use

   Tags

   his-tag (removable with thrombin)

   Expression

   Bacterial

   Mutation

   deleted amino acids 272-297 (encoding the transme…

   Promoter

   T7

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SNAP-Kindlin

   Plasmid

   #186136

   Purpose

   For expression of recombinant Snap-labeled kindlin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   FERMT2 (FERMT2 Human)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB46 (CK2beta)

   Plasmid

   #27085

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRD421

   Plasmid

   #163112

   Purpose

   Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
   

   Use

   Tags

   His-tag with TEV-cleavable linker

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. N-His-GvpC-ssrA

   Plasmid

   #153297

   Purpose

   Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
   

   Use

   Tags

   His-tag and ssrA tag

   Expression

   Bacterial

   Mutation

   1) N-terminal 6xHis tag flanked by 1x glycine res…

   Promoter

   T7

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LARP1_323-509

    Plasmid

    #209800

    Purpose

    Bacterial expression of human LARP1 La-module

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 La-module (LARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-509 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LARP1_323-410

    Plasmid

    #209801

    Purpose

    Bacterial expression of human LARP1 LaM domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 LaM (LARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-410 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)
    

    Use

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. His6-EGFP-PH-PLCD1

    Plasmid

    #183675

    Purpose

    PtdIns(4,5)P2 biosensor for purification from bacteria

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLCD1 (PLCD1 Human)
    

    Use

    Tags

    His6-EGFP

    Expression

    Bacterial

    Mutation

    Amino acids 11-140

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG87 FAST

    Plasmid

    #130719

    Purpose

    Expresses His-tagged FAST (also called YFAST) in bacteria

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    FAST
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. proISG15 (2-165)

    Plasmid

    #110762

    Purpose

    Bacterial expression of proISG15

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Met1 diUb (1-152)

    Plasmid

    #110766

    Purpose

    Bacterial expression of Met1 diUb

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His6-SMT3-SopB

    Plasmid

    #183676

    Purpose

    Recombinant Salmonella Typhimurium SopB

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C-His-GvpC-Calpain

    Plasmid

    #153295

    Purpose

    Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Calpain-sensitive Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) Contains an extra glycine after the methionine…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pINQ-ON10

    Plasmid

    #198689

    Purpose

    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    

    Use

    Tags

    6xHis tag, HA tag, OmpA signal peptide, and Ribos…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pINQ-NbH4

    Plasmid

    #198690

    Purpose

    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody H4
    

    Use

    Tags

    6xHis tag, Avitag, OmpA signal peptide, and Ribos…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge