We narrowed to 1,599 results for: pfas

1. pFBD-Akt13C(WT)

   Plasmid

   #86576

   Purpose

   Expresses cleavable human full-length Akt1(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB1.HMBP.PrS.PHF19

   Plasmid

   #125168

   Purpose

   Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   PHF19 (PHF19 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFBD-Akt13C(S473D)

   Plasmid

   #86577

   Purpose

   Expresses cleavable human full-length Akt1(S473D) phosphomimetic

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130; S473D phos…

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMVS102_P3-GFP-NATMX6

   Plasmid

   #99048

   Purpose

   EGFP reporter with six binding sites for the Zif268 DNA binding domain

   Depositor

   Barak Cohen

   Article

   Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

   Inserts

   McIsaac 2014 P3 promoter
   eGFP
   clonNAT resistance
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL1 promoter with 4 GAL4 sites removed and repla…

   Promoter

   McIsaac 2014 P3 promoter

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac1-GFP(39TAG)

   Plasmid

   #217360

   Purpose

   Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   Enhanced green fluorescent protein
   

   Use

   Baculoviral

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Y39TAG

   Promoter

   CMV

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. R

   Plasmid

   #167170

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-R
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   P10 and PH

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. S

   Plasmid

   #167172

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   P10 and PH

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ADAP1

   Plasmid

   #163905

   Purpose

   Full length mouse ADAP1 for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Use

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB-Flag-MyoD(QQQ)-E12

   Plasmid

   #26003

   Depositor

   Jeffrey Dilworth

   Article

   Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

   Insert

   MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)
   

   Use

   Baculovirus transfer vector

   Tags

   Flag

   Expression

   Mutation

   Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

   Promoter

   Available since

   Sept. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

    Plasmid

    #226884

    Purpose

    Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

    Depositor

    Ning Zheng

    Article

    Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

    Insert

    NaVAb
    

    Use

    Tags

    MBP

    Expression

    Insect

    Mutation

    R4A, N49K, L109A, M116V

    Promoter

    Polyhedrin

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB-D3-ASK1

    Plasmid

    #226619

    Purpose

    Co-expression of D3 and ASK1 complex in insect cells

    Depositor

    Ning Zheng

    Article

    Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

    Inserts

    D3
    ASK1 (SKP1 Mustard Weed)
    

    Use

    Tags

    His tag followed with three copies of Msyb

    Expression

    Insect

    Mutation

    The flexible loop in D3 (Q478-D515) can be remove…

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. KIF13B

    Plasmid

    #163907

    Purpose

    Full length mouse KIF13B for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. KIF13B 1-584

    Plasmid

    #163908

    Purpose

    Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCCM’ CbbQ-

    Plasmid

    #162711

    Purpose

    Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Inactivation of CbbQ subunit of CbbOQ rubisco act…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCM’ ΔacRAF

    Plasmid

    #162710

    Purpose

    Second CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAF

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    pCCM' with a deletion of putative rubisco ch…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y)

    Plasmid

    #155308

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variant

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfAMA1
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    K546M/I547P/I548Y/I549Y

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB1.HMBP.PrS.MTF2

    Plasmid

    #125169

    Purpose

    Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    MTF2 (MTF2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFB1.HMBP.PrS.PHF1

    Plasmid

    #125167

    Purpose

    Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    PHF1 (PHF1 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFB-EPCP5

    Plasmid

    #228879

    Purpose

    Expression of Z-tagged LRRK2 KW using Baculoviruses

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Use

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Amino acids deleted 1-1846

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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