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pcDNA3-Lyso-ExRai-AMPKAR

Plasmid

#192449

Purpose

ExRai-AMPKAR targeted to the lysosomal membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Lyso-ExRai-AMPKAR

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcw107

Plasmid

#62511

Purpose

Gateway Expression Vector

Depositor

John Doench, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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p5E LyzC

Plasmid

#135200

Purpose

Neutrophil specific label

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

Lyz promoter

Use

Synthetic Biology

Promoter

LyzC

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-Ts-His6

Plasmid

#224371

Purpose

Express EF-Ts protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

EF-Ts (tsf E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-G-His8

Plasmid

#224369

Purpose

Express EF-G protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

EF-G (fusA E. coli)

Tags

His8

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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CAG FUCCI

Plasmid

#175266

Purpose

Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

Depositor

James Monaghan

Article

Duerr et al Development. 2022 Mar 15;149(6). pii: 27

Insert

FUCCI

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PB-GFP1-10-HygR

Plasmid

#223675

Purpose

To check the split GFP signals in mitochondria (1)

Depositor

Juan Melero-Martin

Article

Lin et al Nature. 2024 May;629(8012):660-668. doi:

Insert

GFP1-10

Expression

Mammalian

Promoter

cmv

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NUP98_KDM5A

Plasmid

#205872

Purpose

Express mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NUP98_KDM5A (NUP98, KDM5A Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-jAspSnFR3-mRuby3

Plasmid

#203498

Purpose

jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag and mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-Halo-iLID

Plasmid

#190166

Purpose

Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-Halo-iLID

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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