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Plasmid#136450PurposeMammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag YAP S381A
Plasmid#27377DepositorInsertYAP (YAP1 Human)
UseTags2xFLAGExpressionMammalianMutationS381APromoterAvailable sinceMarch 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 FLAG-CBLN2-Fc
Plasmid#115767PurposeExpression of the extracellular domain of CBLN2 with N-terminal FLAG tag and C-terminal hIgG tag.DepositorInsertCBLN2 (CBLN2 Human)
UseTagsFLAG and hIgG/FcExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV Flag PAI-RBP
Plasmid#45452DepositorInsertPAI-RBP (SERBP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR'CMVGFP_hRIF1(1355-2446)IREShygro
Plasmid#23136DepositorInsertRIF1 deletion construct (RIF1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro
Plasmid#154263PurposeDox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.DepositorInsert3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)
UseTags3xFLAGExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV-Beta(4)Integrin
Plasmid#16265DepositorInsertBeta 4 Integrin (ITGB4 Human)
UseTagsExpressionMammalianMutationlacked the 70- and 7-amino acid splice variants a…PromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562A
Plasmid#191976PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorInsertMfn1 T562A (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562APromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562D
Plasmid#191977PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorInsertMfn1 T562D (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562DPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0482-luc2
Plasmid#214635Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0482
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP
Plasmid#194682PurposeCMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_04-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-enCas12a-A3A-Y130F
Plasmid#193652PurposeExpression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLb-enCas12a-hA3A-Y130F, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130FPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FKBP-mTagBFP2-SOScat
Plasmid#178866PurposeMammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2DepositorInsertFKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-DNAJC5 (L115R)
Plasmid#205728PurposeMammalian expression of DNAJC5 (L115R)DepositorInsertDNAJC5 (L115R) (DNAJC5 Human)
UseLentiviralTagsExpressionMutationL115RPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B Flag BimS
Plasmid#23283DepositorInsertpCMV Tag2b Bim S (Bcl2l11 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (TB40/E)
Plasmid#136445PurposeMammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-717). Codon-optimize…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCMV5 Flag-Smad2 EPSM
Plasmid#14933DepositorInsertSmad2 EPSM (SMAD2 Human)
UseTagsFlagExpressionMammalianMutationErk/Pro-directed kinase site mutant (EPSM). T220V…PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 FLAG-BMP2-Fc
Plasmid#115771PurposeExpression of the extracellular domain of BMP2 with N-terminal FLAG tag and C-terminal hIgG tag.DepositorInsertBMP2 (BMP2 Human)
UseTagsFLAG and hIgG/FcExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR'CMVGFP_hRIF1(1701-2446)IREShygro
Plasmid#23137DepositorInsertRIF1 deletion construct (RIF1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f F366H
Plasmid#204141PurposeFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutationDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionMammalianMutationPhenylalanine 366 changed to histidinePromoterCMVAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NLS-FRB-VP16
Plasmid#210515Purposeexpresses NLS-FRB-VP16 component in mammalian cellsDepositorInsertNLS-FRB-VP16
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-A3A-Y132D
Plasmid#193641PurposeExpression of base editor dCas12a-A3A-Y132D in mammalian cells for base editingDepositorInsertdLbCas12a-hA3A-Y132D
UseTagsExpressionMammalianMutationhAPOBEC3A-Y132DPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag
Plasmid#185681PurposeLow-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsHaloTagExpressionMammalianMutationDeletion of lumenal domainPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1P1084A-VSV
Plasmid#139358PurposeExpression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)DepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationP1084APromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc3-Mdm2 p60
Plasmid#52059Purposeexpression of truncated Mdm2 in mammalian cellsDepositorInsertMdm2 p60 (truncated) (MDM2 Human)
UseTagsmyc3ExpressionMammalianMutationp60 (truncated at D367, DVDP motif)PromoterCMVAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-hCas9-T2A-miRFP670
Plasmid#187223PurposeExpresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertCas9
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SPORT6-RNF114-RINGmut
Plasmid#69922PurposeExpresses mutant myc-RNF114 in mammalian cellsDepositorInsertRNF114 (RNF114 Human)
UseTagsExpressionMammalianMutationC29A, C32APromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP
Plasmid#194684PurposeCMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_06-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-eGFP.PB/PTK
Plasmid#170540PurposeExpresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restoredDepositorInsertDelta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2K930R-VSV
Plasmid#139346PurposeExpression of kinase-dead TYK2-K930R proteinDepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-K930RPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (TB40/E)
Plasmid#136453PurposeMammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tagDepositorInsertenvelope glycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (Merlin)
Plasmid#136454PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tagDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-nCas9-RBMX
Plasmid#163565PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-nCas9-RBMX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS2410-Tier1-PhCMV-Myr_mTagBFP2
Plasmid#169535PurposeTier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).DepositorInsertPCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS N354K-I372V
Plasmid#63574PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS N354K-I372V
UseTagsSV40 NLSExpressionMammalianMutationN345K and I372VPromoterCMVAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pShuttle CMV RGECO-TnI
Plasmid#124642PurposeMyofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombinationDepositorInsertRGECO-TnI (TNNI3 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_TOP2
Plasmid#68435PurposeTransient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertTOP2 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAP G116
Plasmid#123099PurposeExpresses 3xFLAG-GABARAP G116 in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAGExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-fRGECO1
Plasmid#125247PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRGECO2
UseTagsNES signalExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only