We narrowed to 7,668 results for: CCH

1. PTPI1-Dsred-URA3

   Plasmid

   #207017

   Purpose

   Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   DsRed-Express 2
   

   Expression

   Yeast

   Promoter

   pTPI1

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hygro-Bem1-mCherry-AID

   Plasmid

   #173925

   Purpose

   Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

   Depositor

   Liedwij Laan

   Article

   Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

   Inserts

   Partial Bem1 C-terminus
   hygromycin marker
   Bem1 downstream sequence
   

   Tags

   mCherry- AID

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF9D94 (pAF10C6)

   Plasmid

   #185848

   Purpose

   Expressing artificial trans-repressor in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PHAC1>TetR>CYC8>PABF1
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

   Plasmid

   #176573

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176577

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Hexahistidine tag and mVenus

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dom34(1-377)

   Plasmid

   #19100

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Tags

   C tag

   Expression

   Bacterial

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

7. Dom34 E23K

   Plasmid

   #19105

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   E23K

   Available Since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

8. Dom34 E149K

   Plasmid

   #19107

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   E149K

   Available Since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

9. Dom34 F218A

   Plasmid

   #19108

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   F218A

   Available Since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

10. Dom34 E261K

    Plasmid

    #19109

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    E261K

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

11. Dom34 Y300A

    Plasmid

    #19110

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    Y300A

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

12. Dom34 K45A

    Plasmid

    #19111

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    K45A

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pCEC-red

    Plasmid

    #196040

    Purpose

    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

    Depositor

    Paola Branduardi

    Article

    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Grna cloning

    Expression

    Yeast

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pREDTAI

    Plasmid

    #51627

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSN075

    Plasmid

    #166732

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cut190*_pET21b

    Plasmid

    #215404

    Purpose

    Expression construct for Cut190* gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Cut190*
    

    Expression

    Bacterial

    Mutation

    S226P/R228S

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. SNM-Luc

    Plasmid

    #35156

    Depositor

    Linda Penn

    Article

    Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

    Insert

    Myc promoter (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains 502 bp of the myc promoter

    Promoter

    c-myc

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry

    Plasmid

    #114322

    Purpose

    Mifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expression

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCLXEBR-pTF-hM3Dq

    Plasmid

    #114283

    Purpose

    TET-inducible expression of the hM3Dq protein

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    hM3Dq
    

    Use

    Lentiviral

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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