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Plasmid#207081PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF169-Halo HRD
Plasmid#207083PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.DepositorInsertHaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-SHLD2 HRD
Plasmid#207078PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNAP-LC3 HRD
Plasmid#207545PurposeHomologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cellsDepositorInsertSNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
UseTagsSNAPtagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG5 HRD
Plasmid#207542PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG5 locus in human cellsDepositorInsertHaloTag flanked by human ATG5 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR knockout HRD
Plasmid#207579PurposeHomologous recombination donor replacing TR with a puro cassette.DepositorInsertpuro resistance cassette flanked by TR locus sequences excluding TR
UseTagsNoneExpressionMammalianMutationPromoterSV40Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-LC3B HRD
Plasmid#207539PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cellsDepositorInsertHaloTag with internal PuroR cassette flanked by human LC3B locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.4Ch (pc2393)
Plasmid#211730PurposeExpresses MBD2 (aa236-414) mCherry tagged in insect cellsDepositorInsertMBD2.4 aa236-414
UseTagsmCherryExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.3Ch (pc2392)
Plasmid#211729PurposeExpresses MBD2 (aa222-414) mCherry tagged in insect cellsDepositorInsertMBD2.3 aa222-414
UseTagsmCherryExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.5R (pc2395)
Plasmid#211727PurposeExpresses MBD2 (aa153-221) RFP tagged in insect cellsDepositorInsertMBD2.5 aa153-221
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.6R (pc2397)
Plasmid#211725PurposeExpresses MBD2 (aa153-235) RFP tagged in insect cellsDepositorInsertMBD2.6 aa153-235
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.1mR (pc2391)
Plasmid#211723PurposeExpresses MBD2 (aa1-152) RFP tagged in insect cellsDepositorInsertMBD2.1 aa1-152
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB_MBD2.2-GFP (pc2083)
Plasmid#211722PurposeExpresses MBD2 (aa153-414) GFP tagged in insect cellsDepositorInsertMBD2.2 aa153-414
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB_MBD2.1-GFP (pc2082)
Plasmid#211721PurposeExpresses MBD2 (aa1-152) GFP tagged in insect cellsDepositorInsertMBD2 aa1-152
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2.9Ch (pc2385)
Plasmid#211720PurposeExpresses MeCP2 (aa163-309) mCherry tagged in insect cellsDepositorInsertMeCP2 aa163-309
UseTagsmCherryExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2G.9 (pc1574)
Plasmid#211719PurposeExpresses MeCP2 (aa163-309) GFP tagged in insect cellsDepositorInsertMeCP2 aa163-309
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2G.8 (pc1573)
Plasmid#211718PurposeExpresses MeCP2 (aa310-492) GFP tagged in insect cellsDepositorInsertMeCP2 aa310-492
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB MBD2a-2-strep (pc1794)
Plasmid#211715PurposeExpresses fl. MBD2 2xStrep tagged in insect cellsDepositorInsertMBD2
UseTags2xStrepExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB MBD2a-C-GFP (pc1565)
Plasmid#211712PurposeExpresses fl. MBD2 GFP tagged in insect cellsDepositorInsertMBD2
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-FL
Plasmid#171602PurposeExpresses full length S. pombe Mdn1 in insect cells.DepositorInsertMdn1
UseTagsHis6 tagExpressionInsectMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only